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Patch-Clamp Analysis

Advanced Techniques

  • Wolfgang Walz
  • Alan A. Boulton
  • Glen B. Baker

Part of the Neuromethods book series (NM, volume 35)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Richard A. Levis, James L. Rae
    Pages 1-34
  3. Harald Sontheimer, Christopher B. Ransom
    Pages 35-67
  4. David J. A. Wyllie
    Pages 69-109
  5. Hartmut Schmidt, Jens Eilers
    Pages 111-134
  6. James R. Moyer Jr., Thomas H. Brown
    Pages 135-193
  7. Raimondo D’Ambrosio
    Pages 195-216
  8. Manfred Heckmann, Christian Pawlu
    Pages 217-229
  9. Gea-Ny Tseng, Minoru Horie
    Pages 231-244
  10. Richard H. Kramer
    Pages 245-264
  11. Blake D. Anson, William M. Roberts
    Pages 265-286
  12. Frances Moody-Corbett, Mark Fry
    Pages 287-299
  13. Gerald Seifert, Albert Becker, Christian Steinhäuser
    Pages 301-330
  14. Back Matter
    Pages 331-345

About this book

Introduction

The use of the patch pipet has moved from a specialized to a mainstream method that can be combined with other techniques to perform complex and novel operations. In Patch-Clamp Analysis: Advanced Techniques, Wolfgang Walz and his colleagues bring together a team of leading academic researchers to describe in reproducible detail all the most important patch-clamp methods. Beginning with updated basic patch-clamp techniques, these hands-on experts present the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose-patch, the perforated-patch, and the patch-cram detection technique. Coverage of these advanced techniques combines their molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques. All the methods included describe the theoretical background, offer complete startup help, and provide tips on solving common problems and analyzing data.
Comprehensive and highly practical, Patch-Clamp Analysis: Advanced Techniques surveys all the major applications that involve patch pipets and sets the stage for the emergence of today's new applications involving genomics, proteomics, and sophisticated imaging techniques.

Editors and affiliations

  • Wolfgang Walz
    • 1
  • Alan A. Boulton
    • 2
  • Glen B. Baker
    • 3
  1. 1.University of SaskatchewanSaskatoonCanada
  2. 2.Alviva Biopharmaceuticals Inc.SaskatoonCanada
  3. 3.University of AlbertaEdmontonCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592592767
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-013-7
  • Online ISBN 978-1-59259-276-0
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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