Gene Probes

Principles and Protocols

  • Marilena Aquino de Muro
  • Ralph Rapley

Part of the Methods in Molecular Biology book series (MIMB, volume 179)

Table of contents

  1. Front Matter
    Pages i-x
  2. Elena Hilario
    Pages 13-18
  3. Elena Hilario
    Pages 19-22
  4. Alex Reid
    Pages 23-25
  5. Alex Reid
    Pages 27-28
  6. Alexis Mendoza-León, Luis Luis, Clara Martínez
    Pages 61-83
  7. Peter Timms, Sarah Mathews
    Pages 93-100
  8. Christopher J. McNamara, Laura G. Leff
    Pages 187-192
  9. John Sanseverino, Alice C. Layton, Gary S. Sayler
    Pages 193-209

About this book

Introduction

The use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions.
Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today.

Editors and affiliations

  • Marilena Aquino de Muro
    • 1
  • Ralph Rapley
    • 2
  1. 1.CABI Bioscience UK CentreSurreyUK
  2. 2.University of HertfordshireUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592592384
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-885-1
  • Online ISBN 978-1-59259-238-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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