Adipose Tissue Protocols

  • Gérard Ailhaud

Part of the Methods in Molecular Biology™ book series (MIMB, volume 155)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Louis Casteilla, Luc Pénicaud, Béatrice Cousin, Denis Calise
    Pages 1-19
  3. Saverio Cinti, M. Cristina Zingaretti, Raffaella Cancello, Enzo Ceresi, Plinio Ferrara
    Pages 21-51
  4. Daniela Malide
    Pages 53-64
  5. Mario DiGirolamo, Jacqueline B. Fine
    Pages 65-75
  6. Hans-Georg Joost, Annette Schürmann
    Pages 77-82
  7. Heidi K. Ortmeyer
    Pages 89-96
  8. Cecilia Holm, Gunilla Olivecrona, Malin Ottosson
    Pages 97-119
  9. Raymond Bazin, Pascal Ferré
    Pages 121-127
  10. Christian Carpéné
    Pages 129-140
  11. Jean-François Tanti, Mireille Cormont, Thierry Grémeaux, Yannick Le Marchand-Brustel
    Pages 157-165
  12. Eva Degerman, Svante Resjö, Tova Rahn Landström, Vincent Manganiello
    Pages 167-180
  13. Susan K. Fried, Naima Moustaid-Moussa
    Pages 197-212

About this book

Introduction

Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded the isolation, subcellular fractionation, and transfection of low density adipocytes, as well as the metabolic aspects of nutrient uptake and key assays of nutrient and ion fluxes. Also covered are: biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT; measurements of adipose secretory products; assessment of WAT metabolism in vivo; and assays of lipid-related enzymes.
Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective nutritional, physiological, and molecular-level research on adipose tissue.

Editors and affiliations

  • Gérard Ailhaud
    • 1
  1. 1.Université de Nice-Sophia AntipolisNiceFrance

Bibliographic information

  • DOI https://doi.org/10.1385/1592592317
  • Copyright Information Humana Press 2001
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-747-2
  • Online ISBN 978-1-59259-231-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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