Posttranslational Modifications of Proteins

Tools for Functional Proteomics

  • Christoph Kannicht

Part of the Methods in Molecular Biology™ book series (MIMB, volume 194)

Table of contents

  1. Front Matter
    Pages i-xi
  2. George N. Saddic, Mary Beth Ebert, Shirish T. Dhume, Kalyan R. Anumula
    Pages 23-36
  3. Martin Gohlke
    Pages 45-61
  4. Christoph Kannicht, Anke Flechner
    Pages 63-72
  5. Monika Rex-Mathes, Jürgen Koch, Sabine Werner, Lee S. Griffith, Brigitte Schmitz
    Pages 73-87
  6. Juan J. Calvete, Libia Sanz
    Pages 89-100
  7. Katalin F. Medzihradszky
    Pages 101-125
  8. Shirish T. Dhume, Mary Beth Ebert, George N. Saddic, Kalyan R. Anumula
    Pages 127-142
  9. Michael Veit, Evgeni Ponimaskin, Michael F. G. Schmidt
    Pages 159-178
  10. Edward G. Mimnaugh, Leonard M. Neckers
    Pages 179-203
  11. Randy J. Arnold, James P. Reilly
    Pages 205-210
  12. Jens R. Bundgaard, Anders H. Johnsen, Jens F. Rehfeld
    Pages 223-239
  13. Gregory P. Mueller, William J. Driscoll
    Pages 241-257
  14. Francis J. Castellino, Victoria A. Ploplis, Li Zhang
    Pages 259-268
  15. Verne Schirch, Sonia Delle Fratte, Martino di Salvo
    Pages 269-276
  16. Mitsuo Yamauchi, Masashi Shiiba
    Pages 277-290

About this book

Introduction

The majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation.
Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.

Editors and affiliations

  • Christoph Kannicht
    • 1
  1. 1.Institut für Molekularbiologie und BiochemieFreie Universität BerlinBerlinGermany

Bibliographic information

  • DOI https://doi.org/10.1385/1592591817
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-678-9
  • Online ISBN 978-1-59259-181-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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