High Throughput Screening

Methods and Protocols

  • William P. Janzen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 190)

Table of contents

  1. Front Matter
    Pages i-x
  2. Ricardo Macarrón, Robert P. Hertzberg
    Pages 1-29
  3. John W. Carpenter, Carmen Laethem, Frederick R. Hubbard, Thomas K. Eckols, Melvyn Baez, Don McClure et al.
    Pages 31-49
  4. James S. Marks, Douglas S. Burdette, David A. Giegel
    Pages 51-63
  5. Patricia Johnston
    Pages 107-116
  6. James A. Chan, Juan A. Hueso-Rodríguez
    Pages 117-127
  7. Wilma W. Keighley, Terry P. Wood
    Pages 129-152
  8. Susan P. Manly, Ramesh Padmanabha, Susan E. Lowe
    Pages 153-168
  9. Steven Hamilton
    Pages 169-189
  10. Karl C. Menke
    Pages 195-211
  11. Seth Cohen, Robert F. Trinka
    Pages 213-228
  12. Back Matter
    Pages 247-265

About this book

Introduction

Since its first use in antibiotic research in the 1940s, High Throughput Screening (HTS) has evolved into a major enabling tool and methodology in the discovery of pharmaceutical, chemical, and agricultural compounds. In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wished had been available when they started in HTS, these seasoned authors illuminate the HTS process with richly detailed tutorials on the biological techniques involved, the management of compound libraries, and the automation and engineering approaches needed. Extensive discussions provide readers with all those key elements of pharmacology, molecular biology, enzymology, and biochemistry that will ensure the identification of suitable targets and screens, and detail the technology necessary to mine millions of data points for meaningful knowledge.
By presenting accounts not only of the basic principles underlying HTS technology, but also real-use cases, High Throughput Screening constitutes a highly practical introduction to HTS, even as it also provides sufficient detail to work more productively in today's lead generation laboratories.

Editors and affiliations

  • William P. Janzen
    • 1
  1. 1.Amphora DiscoveryResearch Triangle Park

Bibliographic information

  • DOI https://doi.org/10.1385/1592591809
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-889-9
  • Online ISBN 978-1-59259-180-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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