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  • © 2001

Meningococcal Vaccines

Methods and Protocols

Part of the book series: Methods in Molecular Medicine (MIMM, volume 66)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvii
  2. Immune Response and Host-Pathogen Interactions

    • Andrew J. Pollard, David Goldblatt
    Pages 23-39
  3. Purification of Capsular Polysaccharide

    • Qingling Yang, Harold Jennings
    Pages 41-47
  4. Protein-Polysaccharide Conjugation

    • Zhongwu Guo, Harold Jennings
    Pages 49-54
  5. N-Propionylation

    • Zhongwu Guo, Harold Jennings
    Pages 55-60
  6. Outer Membrane Protein Purification

    • Carmen Arigita, Wim Jiskoot, Matthijs R. Graaf, Gideon F. A. Kersten
    Pages 61-79
  7. Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease

    • Carl E. Frasch, Loek van Alphen, Johan Holst, Jan T. Poolman, Einar Rosenqvist
    Pages 81-107
  8. Methods for Manipulation of Transferrin-Binding Proteins

    • Leanne M. DeWinter, Anthony B. Schryvers
    Pages 109-120
  9. Construction of porA Mutants

    • Peter van der Ley, Loek van Alphen
    Pages 145-154
  10. Construction of LPS Mutants

    • Peter van der Ley, Liana Steeghs
    Pages 155-165
  11. Recombinant Proteins in Vaccine Development

    • Myron Christodoulides, Keith A. Jolley, John E. Heckels
    Pages 167-180
  12. Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity

    • Alexei A. Delvig, John H. Robinson, Lee Wetzler
    Pages 199-221
  13. Early Experiences with Nasal Vaccines against Meningococcal Disease

    • Johan Holst, Bjørn Haneberg
    Pages 223-239
  14. Animal Models for Meningococcal Disease

    • Andrew R. Gorringe, Karen M. Reddin, Pierre Voet, Jan T. Poolman
    Pages 241-254
  15. Determination of Antibody Responses to Meningococcal Antigens by ELISA

    • Einar Rosenqvist, Helena Käyhty, Andrew J. Pollard
    Pages 255-273

About this book

The development of a comprehensive vaccine against Neisseria meningitidis, the causative agent of meningococcal disease, has remained elusive because of bacterial diversity and immunologic evasion. In Meningococcal Vaccines: Methods and Protocols, Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. They also present as a test case the recent implementation of a new meningococcal vaccine in the United Kingdom. A companion volume, Meningococcal Disease: Methods and Protocols, contains detailed methods for diagnostic microbiology, bacterial characterization, epidemiology, host-pathogen interactions, and clinical studies.
Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus and to develop, evaluate, and implement successful new meningococcal vaccines.

Keywords

  • infectious diseases

Reviews

"This volume is an impressive collection of the latest techniques in the development, evaluation and application of meningococcal vaccines. The easy-to-follow protocols each preceded by a brief introduction into the topic were provided by leading scientists in the field. Andrew Pollard and Martin Maiden did a great job in assembling these protocols into one volume - a work we all in the meningococcal field have waited for." - International Journal of Medical Microbiology.

"The strength of this text is that it provides a key reference source, enabling researchers in the field to avoid reinventing the wheel. The text also provides the basis for the development of new ideas. Each chapter takes a step-by-step approach with clearly set out notes highlighting the problem areas. All the chapters have comprehensive bibliographies, and are well structured. The text is easily accessible and is recommended to those who are seeking to critically appraise the field of meningococcal vaccines." - Epidemiology and Infection

"This book and its accompanying volume are well produced, easy to read and well edited. Andrew Pollard and Martin Maiden are to be congratulated in bringing together in these two volumes an enormous amount of information about this very important topical infection." -Molecular Biotechnology

Editors and Affiliations

  • BC Research Institute for Children’s and Women’s Health, Vancouver, Canada

    Andrew J. Pollard

  • Wellcome Trust Centre for the Epidemiology of Infectious Disease, Oxford, UK

    Martin C.J. Maiden

Bibliographic Information

  • Book Title: Meningococcal Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew J. Pollard, Martin C.J. Maiden

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591485

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • Hardcover ISBN: 978-0-89603-801-1

  • Softcover ISBN: 978-1-4899-4225-8

  • eBook ISBN: 978-1-59259-148-0

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVII, 421

  • Number of Illustrations: 20 b/w illustrations

  • Topics: Diseases

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-148-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 229.00
Price excludes VAT (USA)