Enzymes in Nonaqueous Solvents

Methods and Protocols

  • Evgeny N. Vulfson
  • Peter J. Halling
  • Herbert L. Holland

Part of the Methods in Biotechnology book series (MIBT, volume 15)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Control of Enzyme Activity in Nonaqueous Solvents

    1. Front Matter
      Pages 1-2
    2. Michael T. Ru, Jonathan S. Dordick, Jeffrey A. Reimer, Douglas S. Clark
      Pages 3-11
    3. Joseph O. Rich, Jonathan S. Dordick
      Pages 13-17
    4. Atsuo Tanaka, Takamitsu Iida
      Pages 19-30
    5. Jeffrey A. Khan, Evgeny N. Vulfson
      Pages 31-40
    6. Abu BakarSalleh, Norhaizan M. Esa, Mahiran Basri, Che Nyonya A. Razak, Wan Md Zin W. Yunus, Mansor Ahmad
      Pages 41-48
    7. Ayako Matsushima, Yoh Kodera, Misao Hiroto, Hiroyuki Nishimura, Yuji Inada
      Pages 49-64
    8. Mahiran Basri, Kamaruzaman Ampon, Che Nyonya A. Razak, Abu Bakar Salleh
      Pages 65-75
    9. Francesco Secundo, Gianluca Ottlina, Giacomo Carrea
      Pages 77-81
    10. Barry D. Moore, Johann Partridge, Peter J. Halling
      Pages 97-104
    11. George Bell, Peter J. Halling, Lindsey May, Barry D. Moore, Donald A. Robb, Rein Ulijn et al.
      Pages 105-126
    12. Ernst Wehtje, Patrick Adlercreutz
      Pages 127-134
    13. Joon Shick Rhee, Seok Joon Kwon, Jeong Jun Han
      Pages 135-150
    14. Amélie Ducret, Michael Trani, Robert Lortie
      Pages 173-182
    15. Mikhail Borisover, Vladimir Sirotkin, Dmitriy Zakharychev, Boris Solomonov
      Pages 183-202
    16. Dirk-Jan van Unen, Johan F. J. sEngbersen, David N. Reinhoudt
      Pages 213-225
    17. Johann Partridge, Neil Harper, Barry D. Moore, Peter J. Halling
      Pages 227-234
    18. Marie-Pierre Bousquet, René-Marc Willemot, Pierre Monsan, Emmanuel Boures
      Pages 235-239
  3. Synthetic Applications

    1. Front Matter
      Pages 241-242
    2. Alexandra N. E. Weissfloch, Romas J. Kazlauskas
      Pages 243-259
    3. Didier Rotticci, Jenny Ottosson, Torbjörn Norin, Karl Hult
      Pages 261-276
    4. Gianluca Ottlina, Francesco Secundo, Giorgio Colombo, Giacomo Carrea
      Pages 319-324
    5. Thierry Maugard, Magali Remaud-Simeon, Pierre Monsan
      Pages 325-330
    6. Haralambos Stamatis, Aristotelis Xenakis, Fragiskos N. Kolisis
      Pages 331-338
    7. Takuo Kawamoto, Atsuo Tanaka
      Pages 339-355
    8. Valérie Rolland, René Lazaro
      Pages 357-371
    9. Shui-Tein Chen, Boonyaras Sookkheo, Suree Phutrahul, Kung-Tsung Wang
      Pages 373-400
    10. Shinobu Oda, Takeshi Sugai, Hiromichi Ohta
      Pages 401-416
    11. Andrew J. Smallridge, Maurie A. Trewhella
      Pages 417-422
    12. Brian Morgan, David R. Dodds, Michael J. Homann, Aleksey Zaks, Robert Vail
      Pages 423-467
  4. Reaction Systems and Bioreactor Design

    1. Front Matter
      Pages 469-470
    2. Markus Erbeldinger, Uwe Eichhorn, Peter Kuhl, Peter J. Halling
      Pages 471-477
    3. Cristina Otero, Jose A. Arcos, Hugo S. Garcia, Charles G. Hill Jr.
      Pages 479-496
    4. Sylvain Lamare, Marie Dominique Legoy
      Pages 497-507
    5. Tsuneo Yamane
      Pages 509-516
    6. Haralambos Stamatis, Vasiliki Sereti, Fragiskos N. Kolisis
      Pages 517-522
    7. Marion Haensler, Hans-Dieter Jakubke
      Pages 523-530
    8. Douglas B. Sarney, Evgeny N. Vulfson
      Pages 531-543
    9. David A. MacManus, Anna Millqvist-Fureby, Evgeny N. Vulfson
      Pages 545-552
    10. Nuno Fontes, M. Conceição Almeida, Susana Barreiros
      Pages 565-573
    11. Andrey V. Levashov, Natalia L. Klyachko
      Pages 575-586
    12. Alain Marty, Jean-Stéphane Condoret
      Pages 587-602

About this book


In recent years, enzymatic catalysis in organic solvents-as opposed to aqueous solutions-has gained considerable attention as a powerful new approach to the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design.
Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.

Editors and affiliations

  • Evgeny N. Vulfson
    • 1
  • Peter J. Halling
    • 2
  • Herbert L. Holland
    • 3
  1. 1.Institute of Food ResearchNorwichUK
  2. 2.University of StrathclydeGlasgowUK
  3. 3.Brock University, St. CatharinesOntarioCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-929-2
  • Online ISBN 978-1-59259-112-1
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • Buy this book on publisher's site
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