Enzymes in Nonaqueous Solvents

Methods and Protocols

  • Evgeny N. Vulfson
  • Peter J. Halling
  • Herbert L. Holland

Part of the Methods in Biotechnology book series (MIBT, volume 15)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Control of Enzyme Activity in Nonaqueous Solvents

    1. Front Matter
      Pages 1-2
    2. Michael T. Ru, Jonathan S. Dordick, Jeffrey A. Reimer, Douglas S. Clark
      Pages 3-11
    3. Joseph O. Rich, Jonathan S. Dordick
      Pages 13-17
    4. Atsuo Tanaka, Takamitsu Iida
      Pages 19-30
    5. Jeffrey A. Khan, Evgeny N. Vulfson
      Pages 31-40
    6. Abu BakarSalleh, Norhaizan M. Esa, Mahiran Basri, Che Nyonya A. Razak, Wan Md Zin W. Yunus, Mansor Ahmad
      Pages 41-48
    7. Ayako Matsushima, Yoh Kodera, Misao Hiroto, Hiroyuki Nishimura, Yuji Inada
      Pages 49-64
    8. Mahiran Basri, Kamaruzaman Ampon, Che Nyonya A. Razak, Abu Bakar Salleh
      Pages 65-75
    9. Francesco Secundo, Gianluca Ottlina, Giacomo Carrea
      Pages 77-81
    10. Barry D. Moore, Johann Partridge, Peter J. Halling
      Pages 97-104
    11. George Bell, Peter J. Halling, Lindsey May, Barry D. Moore, Donald A. Robb, Rein Ulijn et al.
      Pages 105-126
    12. Ernst Wehtje, Patrick Adlercreutz
      Pages 127-134
    13. Joon Shick Rhee, Seok Joon Kwon, Jeong Jun Han
      Pages 135-150
    14. Amélie Ducret, Michael Trani, Robert Lortie
      Pages 173-182
    15. Mikhail Borisover, Vladimir Sirotkin, Dmitriy Zakharychev, Boris Solomonov
      Pages 183-202
    16. Dirk-Jan van Unen, Johan F. J. sEngbersen, David N. Reinhoudt
      Pages 213-225

About this book


In recent years, enzymatic catalysis in organic solvents-as opposed to aqueous solutions-has gained considerable attention as a powerful new approach to the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design.
Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.

Editors and affiliations

  • Evgeny N. Vulfson
    • 1
  • Peter J. Halling
    • 2
  • Herbert L. Holland
    • 3
  1. 1.Institute of Food ResearchNorwichUK
  2. 2.University of StrathclydeGlasgowUK
  3. 3.Brock University, St. CatharinesOntarioCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592591124
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-929-2
  • Online ISBN 978-1-59259-112-1
  • Series Print ISSN 1940-6061
  • Series Online ISSN 1940-607X
  • About this book
Industry Sectors
Chemical Manufacturing
Consumer Packaged Goods