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Stress Response

Methods and Protocols

  • John M. Walker
  • Stephen M. Keyse

Part of the Methods in Molecular Biology™ book series (MIMB, volume 99)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Detection and Assay of Stress-Induced Damage

    1. Front Matter
      Pages 1-1
    2. Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, Emily Shacter
      Pages 15-24
    3. Koji Uchida, Earl R. Stadtman
      Pages 25-34
    4. Thomas Reinheckel, Tilman Grune, Kelvin J. A. Davies
      Pages 49-60
  3. The Activation of Signal Transduction by Cellular Stress

    1. Front Matter
      Pages 61-61
    2. D. Grahame Hardie, Ian P. Salt, Stephen P. Davies
      Pages 63-74
    3. Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, Yosef Shiloh
      Pages 99-108
    4. Frank Mercurio, David B. Young, Anthony M. Manning
      Pages 109-125
    5. Ana Cuenda, Dario R. Alessi
      Pages 161-175
    6. Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, Ted R. Hupp
      Pages 177-189
  4. The Analysis of Stress-Induced Gene Expression

    1. Front Matter
      Pages 202-202
    2. Judith M. Mueller, Heike L. Pahl
      Pages 205-216
    3. Anu Mathew, Yanhong Shi, Caroline Jolly, Richard I. Morimoto
      Pages 217-255
    4. Michael W. Bate, Sushma R. Jassal, David W. Brighty
      Pages 277-295
    5. Daniel J. Lavery, Philippe Fonjallaz, Fabienne Fleury-Olela, Ueli Schibler
      Pages 321-345
    6. Harry Frank Abts, Thomas Welss, Kai Breuhahn, Thomas Ruzicka
      Pages 347-366
  5. Analysis of Stress Protein Function

    1. Front Matter
      Pages 367-367
    2. Stefan W. Ryter, Egil Kvam, Rex M. Tyrrell
      Pages 369-391
    3. Brian C. Freeman, Annamieke Michels, Jaewhan Song, Harm H. Kampinga, Richard I. Morimoto
      Pages 393-419
    4. Monika Ehrnsperger, Matthias Gaestel, Johannes Buchner
      Pages 421-429
    5. Rainer Benndorf, Katrin Engel, Matthias Gaestel
      Pages 431-445
  6. Back Matter
    Pages 485-488

About this book

Introduction

In is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research.
Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease

Editors and affiliations

  • John M. Walker
  • Stephen M. Keyse
    • 1
  1. 1.ICRF LaboratoriesUniversity of DundeeUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592590543
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-611-6
  • Online ISBN 978-1-59259-054-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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