Skip to main content
SpringerLink
Log in

Mycobacteria Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Tanya Parish

    1. London School of Hygiene and Tropical Medicine, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Neil G. Stoker

    1. London School of Hygiene and Tropical Medicine, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 101)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (31 protocols)

  1. Green Fluorescent Protein

    • Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, V. Deretic
    Pages 245-260

  2. Expression of Genes in Mycobacteria

    • Peadar Ó Gaora
    Pages 261-273

  3. Secretion of Mammalian Proteins from Mycobacteria

    • Peter J. Murray, Richard A. Young
    Pages 275-284

  4. Intracellular Gene Expression

    • Philip D. Butcher, Joseph A. Mangan, Irene M. Monahan
    Pages 285-306

  5. 24 Analysis of Mycobacterial Differential Gene Expression by RAP-PCR

    • Joseph A. Mangan, Philip D. Butcher
    Pages 307-322

  6. Mycobacterial Speciation

    • Francis A. Drobniewski, Anne H. C. Uttley
    Pages 323-347

  7. Species Identification of Mycobacteria Using rDNA Sequencing

    • Philip Kirschner, Erik C. Bottger
    Pages 349-361

  8. Detection of Mycobacterium tuberculosis by a Calorimetric Polymerase Chain Reaction

    • Stuart M. Wilson
    Pages 363-380

  9. Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping

    • Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, Jan D. A. van Embden
    Pages 381-394

  10. Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis

    • Herre F. Heersma, Kristin Kremer, Jan D. A. van Embden
    Pages 395-422

  11. Detection of Mutations in Mycobacteria by PCR-SSCP (Single-Strand Conformation Polymorphism)

    • Amalio Telenti, Nadine Honoré, Stewart T. Cole
    Pages 423-430

  12. The Use of Luciferase-Reporter Phage for Antibiotic-Susceptibility Testing of Mycobacteria

    • Paul F. Riska, William R. Jacobs Jr.
    Pages 431-455

  13. Back Matter

    Pages 457-472
    Download chapter PDF
Back to top

About this book

Mycobacteria include species of great medical importance, being - sponsible for both human and animal disease, including tuberculosis and lep- . The use of molecular techniques to explore the biology of these organisms rosy has exploded in the past few years. Mycobacteria pose particular problems in the laboratory, most notably their slow growth rate, their tendency to clump, and their resistance to standard methods for lysis; thus special adaptations of provides - commonly used methods are required . Mycobacteria Protocols scriptions of both basic and recently developed techniques for studying the molecular biology of mycobacteria. Although many genetic tools are still - quired, there is much that can be accomplished using those available, and this book provides a starting point for anyone interested in working on these - triguing, but difficult bacteria . The first eight chapters describe the basic techniques required for any study of mycobacteria, including culture and safety aspects, and preparation of nucleic acids, proteins, and cell-wall fractions. The introduction of DNA into mycobacteria was a major advance in enabling the development of - netic systems. Two chapters deal with introduction of DNA by electroporation and conjugation. Phages have also been used as the basis for genetic systems, . and a comprehensive list of protocols for their use is presented It is becoming difficult to handle the rapidly increasing body of kno- edge in the mycobacterial field.

Reviews

"...this book is an essential purchase for an laboratory dealing with mycobacteria and good value for individual researchers."
-Microbiology Today

"...a unique book...presents excellent description of many of the molecular biological tools that have been developed...an invaluable collection of methodologies for mycobacterial research...the molecular mycobacteriologist will find much of interest in this book...Worth getting." -Journal of Medical Microbiology

"...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms...of interest to the epidemiologist is the description of computer analysis of DNA fingerprinting for tuberculosis while the chapter on accessing MycDB, a database dedicated to mycobacterial research, will be of value to all those working in this area."-Molecular Biotechnology

"...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms....The detailed technical nature of each chapter provides clear instruction for those wishing to engage in this area of research." - Molecular Biotechnology

Editors and Affiliations

  • London School of Hygiene and Tropical Medicine, London, UK

    Tanya Parish, Neil G. Stoker

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation