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Plant Virology Protocols

From Virus Isolation to Transgenic Resistance

  • Book
  • © 1998

Overview

Editors:
  1. Gary D. Foster

    1. Plant Virology Group School of Biological Sciences, University of Bristol, UK
      Department of Botany, University of Leicester, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Sally C. Taylor

    1. Department of Botany, University of Leicester, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 81)

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Table of contents (55 protocols)

  1. Virus Isolation, RNA Extraction, and Location of Coat Protein Genes

    1. Cucumovirus Isolation and RNA Extraction

      • Marilyn J. Roossinck, P. Scott White
      Pages 189-196

    2. Nepovirus Isolation and RNA Extraction

      • K. Rogers Wood
      Pages 197-204

    3. Comovirus Isolation and RNA Extraction

      • Joan Wellink
      Pages 205-209

    4. Carmovirus Isolation and RNA Extraction

      • Juana Díez, Jose F. Marcos, Vicente Pallás
      Pages 211-217

    5. Tymovirus Isolation and Genomic RNA Extraction

      • Adrian Gibbs, Anne M. Mackenzie
      Pages 219-224

    6. Tombusvirus Isolation and RNA Extraction

      • József Burgyán, Marcello Russo
      Pages 225-230

    7. Luteovirus Isolation and RNA Extraction

      • Keith L. Perry, Jane Miller, Richard M. Lister, Mike A. Mayo
      Pages 231-239

  2. Cloning of Coat Protein Genes

    1. Front Matter

      Pages 241-241
      Download chapter PDF

    2. RNA Analysis

      • Michael Shanks, George Lomonossoff
      Pages 243-249

    3. RNA Fractionation by Density Gradient Centrifugation

      • Michael Shanks
      Pages 251-254

    4. cDNA Library Construction for the Lambda ZAP®-Based Vectors

      • Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short
      Pages 255-267

    5. PCR Cloning of Coat Protein Genes

      • Rebecca Stratford
      Pages 269-278

    6. Antibody Production

      • Carol Brattey, Robert Burns
      Pages 279-286

    7. Expression Library Screening

      • Gary D. Foster
      Pages 287-291

    8. In Vitro Transcription and Translation

      • Roisin Turner, Gary D. Foster
      Pages 293-299

    9. Analysis of Coat Protein Expression Cassettes in Protoplasts

      • Jonathan H. Weston, Roisin L. Turner
      Pages 301-305

    10. DNA Sequencing

      • Jo Badge
      Pages 307-318

    11. Computer Analysis of Amino Acid Sequences

      • Eugene V. Koonin, Arcady R. Mushegian, Valerian V. Dolja
      Pages 319-337

  3. Plant Transformation and Primary Analysis

    1. Front Matter

      Pages 339-339
      Download chapter PDF

    2. Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation

      • Corrin V. Wallis, Margaret I. Boulton
      Pages 341-352
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About this book

The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.

Editors and Affiliations

  • Plant Virology Group School of Biological Sciences, University of Bristol, UK

    Gary D. Foster

  • Department of Botany, University of Leicester, UK

    Gary D. Foster, Sally C. Taylor

Bibliographic Information

  • Book Title: Plant Virology Protocols

  • Book Subtitle: From Virus Isolation to Transgenic Resistance

  • Editors: Gary D. Foster, Sally C. Taylor

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033856

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • Hardcover ISBN: 978-0-89603-385-6Published: 24 February 1998

  • Softcover ISBN: 978-1-4899-4253-1Published: 14 August 2013

  • eBook ISBN: 978-1-59259-566-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 571

  • Number of Illustrations: 111 b/w illustrations

  • Topics: Plant Sciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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