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  • Book
  • Jun 1995

Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 44)

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Table of contents (33 protocols)

  1. Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells

    • Nello Bagni, Marisa Mengoli, Shigeru Matsuzaki
    Pages 223-236
  2. IAA Analysis in Transgenic Plants

    • John F. Hall, Sarah J. Brown, Kevan M. A. Gartland
    Pages 237-244
  3. Quantifying Phytohormones in Transformed Plants

    • Els Prinsen, Pascale Redig, Miroslav Strnad, Ivan Galís, Walter Van Dongen, Henri Van Onckelen
    Pages 245-262
  4. Manipulating Photosynthesis in Transgenic Plants

    • Jacqueline S. Knight, Francisco Madueño, Simon A. Barnes, John C. Gray
    Pages 263-280
  5. Gene Activation by T-DNA Tagging

    • Klaus Fritze, Richard Walden
    Pages 281-294
  6. Agroinfection

    • Nigel Grimsley
    Pages 325-342
  7. T-DNA Transfer to Maize Plants

    • Wen-Hui Shen, Jesús Escudero, Barbara Hohn
    Pages 343-350
  8. Regulation of Agrobacterium Gene Manipulation

    • Frank Dewhurst
    Pages 369-391
  9. Transgenic Oilseed Rape

    • Alain Baranger, Marie-Claire Kerlan, Anne-Marie Chèvre, Frédérique Eber, Patrick Vallée, Michel Renard
    Pages 393-403
  10. Electroporation Protocols for Agrobacterium

    • Garry D. Main, Stephen Reynolds, Jill S. Gartland
    Pages 405-412
  11. Back Matter

    Pages 413-417

About this book

Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants.

Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.

Editors and Affiliations

  • Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  • Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

Bibliographic Information

  • Book Title: Agrobacterium Protocols

  • Editors: Kevan M. A. Gartland, Michael R. Davey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033023

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1995

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 417

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma