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Antibody Engineering Protocols

  • Book
  • © 1995

Overview

Editors:
  1. Sudhir Paul

    1. Department of Anesthesiology, University of Nebraska Medical Center, Omaha

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Part of the book series: Methods in Molecular Biology (MIMB, volume 51)

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Table of contents (30 protocols)

  1. Cloning and Bacterial Expression of an Esterolytic sFv

    • Rodger G. Smith, Mark T. Martin, Rosa Sanchez, John H. Kenten
    Pages 297-317

  2. Site-Directed Mutagenesis of Antibody-Variable Regions

    • Qing-Sheng Gao, Sudhir Paul
    Pages 319-327

  3. Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation

    • Su-jun Deng, C. Roger MacKenzie, Saran A. Narang
    Pages 329-342

  4. Chaperonins in Phage Display of Antibody Fragments

    • Eskil Söderlind, Marta Dueñas, Carl A. K. Borrebaeck
    Pages 343-353

  5. Phage-Display Libraries of Murine and Human Antibody Fab Fragments

    • Jan Engberg, Peter Sejer Andersen, Leif Kofoed Nielsen, Morten Dziegiel, Lene K. Johansen, Bjarne Albrechtsen
    Pages 355-376

  6. Selection of Human Immunoglobulin Light Chains from a Phage-Display Library

    • Sonia Tyutyulkova, Qing-Sheng Gao, Sudhir Paul
    Pages 377-394

  7. Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography

    • Sonia Tyutyulkova, Sudhir Paul
    Pages 395-401

  8. Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening

    • Han Huang, Brian Fichter, Robert Dannenbring, Sudhir Paul
    Pages 403-407

  9. Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies

    • Han Huang, Sudhir Paul
    Pages 409-415

  10. Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies

    • Lan Li, Ravishankar Kalaga, Srinivas Kaveri, Sudhir Paul
    Pages 417-421

  11. Radiolabeling of Antibodies for Therapy and Diagnosis

    • Janina Baranowska-Kortylewicz, Glenn V. Dalrymple, Syed M. Quadri, Katherine A. Harrison
    Pages 423-439

  12. Back Matter

    Pages 441-450
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Editors and Affiliations

  • Department of Anesthesiology, University of Nebraska Medical Center, Omaha

    Sudhir Paul

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