PCR Protocols

1. Sequencing DNA Amplified Directly from a Bacterial Colony

   • Martin A. Hofmann, David A. Brian

   Pages 205-210

2. Use of Polymerase Chain Reaction to Screen Phage Libraries

   • Lei Yu, Laura J. Bloem

   Pages 211-215

3. Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends

   • Beverly C. Delidow

   Pages 217-228

4. Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products

   • Alan R. Shuldiner, Keith Tanner

   Pages 229-239

5. Use of Polymerase Chain Reaction for Making Recombinant Constructs

   • Douglas H. Jones, Stanley C. Winistorfer

   Pages 241-250

6. In Vitro Recombination and Mutagenesis of DNA

   • Robert M. Horton

   Pages 251-261

7. Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes

   • Patrick J. Dillon, Craig A. Rosen

   Pages 263-268

8. Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis

   • Douglas H. Jones

   Pages 269-276

9. Site-Directed Mutagenesis by Double Polymerase Chain Reaction

   • Sailen Barik

   Pages 277-286

10. Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA

    • W. Michael Kuehl, James Battey

    Pages 287-304

11. PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy

    • David L. Cooper, Narayana R. Isola

    Pages 305-316

12. Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members

    • Gregory M. Preston

    Pages 317-337

13. Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking

    • Venkatakrishna Shyamala, Giovanna Ferro-Luzzi Ames

    Pages 339-348

14. cDNA Cloning by Inverse Polymerase Chain Reaction

    • Sheng-He Huang, Chun-Hua Wu, Bing Cai, John Holcenberg

    Pages 349-356

15. Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity

    • Sheng-He Huang, Ambrose Y. Jong, Wu Yang, John Holcenberg

    Pages 357-363

16. Anchoring a Defined Sequence to the 5′ Ends of mRNAs

    • Jean Baptiste, Dumas Milne Edwards, Jacques Delort, Jacques Mallet

    Pages 365-385

17. Back Matter

    Pages 387-392

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PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

...affords many new insights and contributions of the first order...an excellent book of the highest interest.-Cellular and Molecular Biology

• University of Connecticut Health Center, Farmington

  Bruce A. White

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