Enzymes of Molecular Biology

1. Front Matter

   Pages i-x

   PDF

2. Nucleases

   • A. Fred Weir

   Pages 1-6

3. Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)

   • A. Fred Weir

   Pages 7-16

4. DNA Polymerases (EC 2.7.7.7)

   • Martin J. Maunders

   Pages 17-30

5. Taq Polymerase (EC 2.7.7.7)

   • Axel Landgraf, Heiner Wolfes

   Pages 31-58

6. Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6)

   • Deborah A. Cook, Robert J. Slater

   Pages 59-72

7. Reverse Transcriptase (EC 2.7.7.49)

   • Gary F. Gerard, James M. D’Alessio

   Pages 73-93

8. Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31)

   • Frank Grosse, Andreas Manns

   Pages 95-105

9. Restriction Enzymes

   • Alfred Pingoud, Jürgen Alves, Robert Geiger

   Pages 107-200

10. DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73)

    • David P. Hornby

    Pages 201-211

11. DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3)

    • Martin J. Maunders

    Pages 213-230

12. The BAL 31 Nucleases (EC 3.1.11)

    • Horace B. Gray Jr., Tao Lu

    Pages 231-251

13. Mung-Bean Nuclease 1 (EC 3.1.30.1)

    • Andrew J. Sharp, Robert J. Slater

    Pages 253-261

14. RNase A (EC 3.1.27.5)

    • Michael M. Burrell

    Pages 263-270

15. Pronase (EC 3.4.24.4)

    • Patricia J. Sweeney, John M. Walker

    Pages 271-276

16. Proteolytic Enzymes for Peptide Production

    • Patricia J. Sweeney, John M. Walker

    Pages 277-303

17. Proteinase K (EC 3.4.21.14)

    • Patricia J. Sweeney, John M. Walker

    Pages 305-311

18. Carboxypeptidase Y (EC 3.4.16.1)

    • Julia S. Winder, John M. Walker

    Pages 313-318

19. Aminopeptidases

    • Patricia J. Sweeney, John M. Walker

    Pages 319-329

20. Alkaline Phosphatase (EC 3.1.3.1)

    • Martin J. Maunders

    Pages 331-341

The scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.

An essential resource for every biochemist and scientist in molecular biology. -Unlisted Drugs

• Advanced Technologies (Cambridge) Limited, Cambridge, UK

  Michael M. Burrell

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