Pyrosequencing® Protocols

  • John M. Walker
  • Sharon Marsh

Part of the Methods in Molecular Biology™ book series (MIMB, volume 373)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Pål Nyrén
    Pages 1-13
  3. Sharon Marsh
    Pages 15-23
  4. Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos et al.
    Pages 25-38
  5. Cristi R. King, Tiffany Scott-Horton
    Pages 39-55
  6. Dong-Chuan Guo, Dianna M. Milewicz
    Pages 57-62
  7. Jonathon Wasson
    Pages 63-74
  8. Pritesh Patel, Yih-Horng Shiao, Paolo Fortina
    Pages 75-87
  9. Neil Woodford, Sarah E. North, Matthew J. Ellington
    Pages 103-114
  10. Steven Ringquist, Alexis Styche, William A. Rudert, Massimo Trucco
    Pages 115-133
  11. Gerli Pielberg, Leif Andersson
    Pages 147-155
  12. Derek J. Van Booven
    Pages 177-186
  13. Back Matter
    Pages 187-196

About this book


Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.


Allele DNA Data storage Primer selection Pyrosequencing technique Throughput gene expression genes

Editors and affiliations

  • John M. Walker
  • Sharon Marsh
    • 1
  1. 1.Division of OncologyWashington University School of MedicineSt. Louis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-645-0
  • Online ISBN 978-1-59745-377-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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