High Content Screening

A Powerful Approach to Systems Cell Biology and Drug Discovery

  • D. Lansing Taylor
  • Jeffrey R. Haskins
  • Kenneth A. Giuliano

Part of the Methods in Molecular Biology book series (MIMB, volume 356)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction to High Content Screening

  3. Instrumentation, Biological Application Software, and Sample Preparation

    1. Front Matter
      Pages 39-39
    2. Richik N. Ghosh, Oleg Lapets, Jeffrey R. Haskins
      Pages 63-81
    3. Anne L. Plant, John T. Elliott, Alessandro Tona, Dennis McDaniel, Kurt J. Langenbach
      Pages 95-107
    4. Gillian R. Richards, Julie E. Kerby, Grace K. Y. Chan, Peter B. Simpson
      Pages 109-119
    5. Daniel R. Marshak, Dale E. Greenwalt
      Pages 121-128
    6. Oren Beske, Daniel Bassoni, Simon Goldbard
      Pages 129-138
  4. Reagents

    1. Front Matter
      Pages 139-139
    2. Kenneth A. Giuliano, D. Lansing Taylor, Alan S. Waggoner
      Pages 141-163
    3. Georgyi V. Los, Keith Wood
      Pages 195-208
    4. Thomas Machleidt, Matt Robers, George T. Hanson
      Pages 209-220
    5. Marnie L. MacDonald, John K. Westwick
      Pages 221-232
    6. Michael J. Ignatius, Jeffrey T. Hung
      Pages 233-244
    7. K. Gregory Moore, Wayne Speckmann, Ronald P. Herzig
      Pages 245-251
    8. Peter G. Conrad II, Rajesh V. Chavli, Richard S. Givens
      Pages 253-265
  5. Informatics and Bioinformatics

    1. Front Matter
      Pages 267-267
    2. R. Terry Dunlay, Wallace J. Czekalski, Mark A. Collins
      Pages 269-280
    3. Matthew J. Anstett
      Pages 301-317
    4. Sean Ekins, Yuri Nikolsky, Andrej Bugrim, Eugene Kirillov, Tatiana Nikolskaya
      Pages 319-350
  6. Assays and Applications of High Content Screening

    1. Front Matter
      Pages 351-351
    2. Jackie L. Stilwell, Yinghui Guan, Richard M. Neve, Joe W. Gray
      Pages 353-365
    3. Robert A. Blake
      Pages 367-377
    4. Andreas Vogt, John S. Lazo
      Pages 389-400
    5. Frosty Loechel, Sara Bjørn, Viggo Linde, Morten Præstegaard, Len Pagliaro
      Pages 401-414
    6. Peter O’Brien, Jeffrey R. Haskins
      Pages 415-425
    7. John B. Kerrison, Donald J. Zack
      Pages 427-434
  7. Back Matter
    Pages 435-444

About this book


High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery discusses its use as a high throughput platform to understand the functions of genes, RNA, proteins, and other cellular constituents at the level of the living cell.
High Content Screening is assembled to assist both existing users of HCS, as well as investigators considering the addition of a discovery-driven platform to their research and development activities. The chapters have been organized into sections that highlight the importance of integrating instrumentation, application software, reagents, and informatics. In addition, there is a combination of pure review chapters on key topics and specific methods chapters.


bioinformatics biology cell biology cell lines genes proteins siRNA

Editors and affiliations

  • D. Lansing Taylor
    • 1
  • Jeffrey R. Haskins
    • 2
  • Kenneth A. Giuliano
    • 1
  1. 1.Cellumen, Inc.Pittsburgh
  2. 2.Cellomics, Inc.Pittsburgh

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-731-0
  • Online ISBN 978-1-59745-217-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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