Protein Engineering Protocols

  • Katja M. Arndt
  • Kristian M. Müller

Part of the Methods in Molecular Biology™ book series (MIMB, volume 352)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Design and Computational Strategies for Protein Engineering

    1. Front Matter
      Pages 1-1
    2. Hidetoshi Kono, Wei Wang, Jeffery G. Saven
      Pages 3-22
    3. Jamie M. Bacher, Andrew D. Ellington
      Pages 23-34
    4. Jody M. Mason, Kristian M. Müller, Katja M. Arndt
      Pages 35-70
    5. Kevin Truong, Asako Sawano, Atsushi Miyawaki, Mitsuhiko Ikura
      Pages 71-82
    6. Wataru Nomura, Yukio Sugiura
      Pages 83-93
    7. Akiko Koide, Shohei Koide
      Pages 95-109
    8. Peter Friedhoff, Alfred Pingoud
      Pages 111-123
  3. Evolutionary Strategies for Protein Engineering

    1. Front Matter
      Pages 125-125
    2. Michel Denault, Joelle N. Pelletier
      Pages 127-154
    3. Luke H. Bradley, Yinan Wei, Peter Thumfort, Christine Wurth, Michael H. Hecht
      Pages 155-166
    4. Sabine C. Stebel, Katja M. Arndt, Kristian M. Müller
      Pages 167-190
    5. Peter L. Bergquist, Moreland D. Gibbs
      Pages 191-204
    6. Sachdev S. Sidhu, Birte K. Feld, Gregory A. Weiss
      Pages 205-219
    7. Satoshi Fujita, Jing-Min Zhou, Kazunari Taira
      Pages 221-236
    8. Farid J. Ghadessy, Philipp Holliger
      Pages 237-248
    9. Jochen Hecky, Jody M. Mason, Katja M. Arndt, Kristian M. Müller
      Pages 275-304
  4. Back Matter
    Pages 305-312

About this book


Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering.
The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented.
Following the successful Methods in Molecular Biology™ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.


Activation Amino acid Calcium DNA Elongation Nucleotide biochemistry enzymes escherichia coli genes molecular biology protein structure proteins

Editors and affiliations

  • Katja M. Arndt
    • 1
  • Kristian M. Müller
    • 1
  1. 1.Institut für Biologie IIIUniversität FreiburgFreiburgGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-072-4
  • Online ISBN 978-1-59745-187-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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