YAC Protocols

  • Alasdair MacKenzie

Part of the Methods in Molecular Biology™ book series (MIMB, volume 349)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Kerry Miller, Scott Davidson, Alasdair MacKenzie
    Pages 41-59
  3. Shigeki Kawakami, Satoshi Harashima, Akio Kobayashi, Kiichi Fukui
    Pages 61-65
  4. Sanbing Shen, Anthony Harmar, Nick Hastie
    Pages 67-74
  5. Yeon-Hee Kim, Minetaka Sugiyama, Yoshinobu Kaneko, Kiichi Fukui, Akio Kobayashi, Satoshi Harashima
    Pages 103-115
  6. David Markie, Emma Jones, Jiannis Ragoussis
    Pages 117-126
  7. Pedro N. Moreira, Julio Pozueta, Patricia Giraldo, Alfonso Gutiérrez-Adán, Lluís Montoliu
    Pages 151-161
  8. Márcia R. M. Santos, Roberto R. M. Barros, Patrício E. Porcile, Mariano J. Levin, José Franco da Silveira
    Pages 187-197
  9. Back Matter
    Pages 199-203

About this book


It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.


DNA Southern Blot YAC cell lines chromosome gene expression genes genetics hybridization recombination transgenic animals

Editors and affiliations

  • Alasdair MacKenzie
    • 1
  1. 1.School of Medical Sciences, College of Life Sciences and MedicineUniversity of Aberdeen, Institute of Medical SciencesForesterhill, AberdeenScotland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-612-2
  • Online ISBN 978-1-59745-158-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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