Agrobacterium Protocols Volume 2

  • Kan Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 344)

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. Root Plants

    1. Front Matter
      Pages 1-1
    2. Owen Wally, Jayaraj Jayaraman, Zamir K. Punja
      Pages 3-12
    3. Weston Msikita, Uzoma Ihemere, Dimuth Siritunga, Richard T. Sayre
      Pages 13-24
    4. Steve Millam
      Pages 25-35
    5. Guo-qing Song, Ken-ichi Yamaguchi
      Pages 37-44
  3. Turf Grasses

    1. Front Matter
      Pages 45-45
    2. Yaxin Ge, Zeng-Yu Wang
      Pages 47-53
    3. Fredy Altpeter
      Pages 55-64
    4. Mariya N. Somleva
      Pages 65-74
    5. Yaxin Ge, Zeng-Yu Wang
      Pages 75-81
    6. Chip Longo, Colin Lickwar, Qian Hu, Kimberly Nelson-Vasilchik, David Viola, Joel Hague et al.
      Pages 83-95
  4. Woody Species

    1. Front Matter
      Pages 97-97
    2. Andrew E. Newhouse, Franziska Schrodt, Charles A. Maynard, William A. Powell
      Pages 99-112
    3. Rubén Álvarez, Mariano Toribio, Millán Cortizo, Ricardo-Javier Ordás Fernández
      Pages 113-123
    4. Zenn-Zong Chen, Cheng-Kuen Ho, In-Suk Ahn, Vincent L. Chiang
      Pages 125-134
    5. Jan Grant, Tracy Dale, Pauline Cooper
      Pages 135-141
    6. Richard Meilan, Caiping Ma
      Pages 143-151
    7. Perumal Venkatachalam, Radha Jayashree, Karumamkandathil Rekha, Sreedharannair Sushmakumari, Sankaren Sobha, Parukkuttyamma Kumari Jayasree et al.
      Pages 153-164
  5. Tropic Plants

    1. Front Matter
      Pages 165-165
    2. Juan B. Pérez, Serge Remy, Rony Swennen, László Sági
      Pages 167-175
    3. Vladimir Orbović, Jude W. Grosser
      Pages 177-189
    4. Thierry Leroy, Edgardo Alpizar, Magali Dufour, Hervé Etienne
      Pages 191-208
    5. Yun J. Zhu, Maureen M. M. Fitch, Paul H. Moore
      Pages 209-217
    6. Lourdes Yabor, Patricia Espinosa, Ariel D. Arencibia, José C. Lorenzo
      Pages 219-226
    7. Ariel D. Arencibia, Elva R. Carmona
      Pages 227-235
  6. Nuts and Fruits

    1. Front Matter
      Pages 237-237
    2. Charles A. Maynard, Linda D. Polin, Sharon L. LaPierre, Ronald E. Rothrock, William A. Powell
      Pages 239-251
    3. Abhaya M. Dandekar, Gianni Teo, Sandra L. Uratsu, David Tricoli
      Pages 253-261
    4. Guo-Qing Song, Kenneth C. Sink
      Pages 263-272
    5. Alain Bouquet, Laurent Torregrosa, Pat Iocco, Mark R. Thomas
      Pages 273-285
    6. Bruno Mezzetti, Elisa Costantini
      Pages 287-295
    7. Charles A. Leslie, Sandra L. Uratsu, Gale McGranahan, Abhaya M. Dandekar
      Pages 297-307
  7. Ornamental Plants

    1. Front Matter
      Pages 309-309
    2. Chalermsri Nontaswatsri, Seiichi Fukai
      Pages 311-320
    3. Jaime A. Teixeira da Silva
      Pages 321-329
    4. Ming-Tsair Chan, Yuan-Li Chan, Sanjaya
      Pages 331-338
    5. W. Kevin Lutke
      Pages 339-349
    6. Schuyler S. Korban, Ksenija Gasic, Xiangqian Li
      Pages 351-358
  8. Medicinal Plants

    1. Front Matter
      Pages 359-359
    2. Yong Eui Choi
      Pages 361-371
    3. Mistianne Feeney, Zamir K. Punja
      Pages 373-382
    4. Julie A. Chitty, Robert S. Allen, Philip J. Larkin
      Pages 383-391
  9. Non-Plants

    1. Front Matter
      Pages 393-393
    2. Clarence I. Kado, Brian Kelly
      Pages 395-401
    3. Chang Hyun Khang, Sook-Young Park, Hee-Sool Rho, Yong-Hwan Lee, Seogchan Kang
      Pages 403-420
    4. Manchikatla V. Rajam, S. Vinod Kumar
      Pages 421-433
    5. Tzvi Tzfira, Talya Kunik, Yedidya Gafni, Vitaly Citovsky
      Pages 435-451
    6. C. Peter Romaine, Carl Schlagnhaufer
      Pages 453-463
    7. Paul J. J. Hooykaas, Amke den Dulk-Ras, Paul Bundock, Jalal Soltani, Haico van Attikum, G. Paul H. van Heusden
      Pages 465-473

About this book


Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.


DNA Flora algae arabidopsis thaliana fungi genetic engineering molecular biology saccharomyces cerevisiae

Editors and affiliations

  • Kan Wang
    • 1
    • 2
  1. 1.Center for Plant Transformation, Plant Science InstituteIowa State UniverstiyAmes
  2. 2.Department of AgronomyIowa State UniverstiyAmes

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-843-0
  • Online ISBN 978-1-59745-131-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods