Gene Mapping, Discovery, and Expression

Methods and Protocols

  • Minou Bina

Part of the Methods in Molecular Biology book series (MIMB, volume 338)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Razi Khaja, Jeffrey R. MacDonald, Junjun Zhang, Stephen W. Scherer
    Pages 9-20
  3. Avirachan T. Tharapel, Stephen S. Wachtel
    Pages 59-67
  4. Christoph Dieterich, Martin Vingron
    Pages 105-118
  5. Vinsensius Berlian Vega
    Pages 119-127
  6. Minou Bina, Phillip Wyss, Syed Rehan Shah
    Pages 129-134
  7. Phillip Wyss, Sheryl A. Lazarus, Minou Bina
    Pages 135-152
  8. Julian C. Knight
    Pages 153-165
  9. Yoko Fukuda, Hiroaki Kawasaki, Kazunari Taira
    Pages 167-173
  10. Jeremy Gollub, Catherine A. Ball, Gavin Sherlock
    Pages 191-208
  11. Tomara.J. Fleury, Alfred Cioffi, Arnold Stein
    Pages 209-223
  12. Scott A. Hoose, Michael P. Kladde
    Pages 225-244
  13. Jiannis Ragoussis, Simon Field, Irina A. Udalova
    Pages 261-279
  14. Mariana Yaneva, Paul Tempst
    Pages 291-303
  15. Patrick Rodriguez, Harald Braun, Katarzyna E. Kolodziej, Ernie de Boer, Jennifer Campbell, Edgar Bonte et al.
    Pages 305-323
  16. Back Matter
    Pages 325-334

About this book


Completion of the sequence of the human genome and the development of high-throughput technologies have opened a new era of research in biology and medicine. In Gene Mapping, Discovery, and Expression: Methods and Protocols, a panel of researchers from around the world and from a variety of disciplines offer a unique blend of computational tools and experimental techniques for gene mapping, comparative genomics, and studies of the control of gene expression. On the computational side, for beginners in the field, the book describes web-based technologies to localize and map genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential "codes" in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis and characterization of transcription factor binding elements, and methods for isolation and identification of proteins that interact with DNA. The protocols in the book follow the successful Methods in Molecular Biology™ series format, offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.
Cutting-edge and interdisciplinary, Gene Mapping, Discovery, and Expression: Methods and Protocols offers a collection of DNA-sequence-based technologies for gene discovery, retrieval of gene expression data, discovery of regulatory elements, and discovery of transcription factors that interact with DNA.


Allele DNA Microarray Promoter gene expression genes molecular biology transcription

Editors and affiliations

  • Minou Bina
    • 1
  1. 1.Department of ChemistryPurdue UniversityWest LafayetteUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-575-0
  • Online ISBN 978-1-59745-097-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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