Transmembrane Signaling Protocols

  • Hydar Ali
  • Bodduluri Haribabu

Part of the Methods in Molecular Biology™ book series (MIMB, volume 332)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Overviews

  3. Specific Topics

    1. Front Matter
      Pages 101-101
    2. Membrane Receptors and Signaling

      1. Marcin Majka, Mariusz Z. Ratajczak
        Pages 103-114
      2. Jean-Marc Navenot, Zi-xuan Wang, Stephen C. Peiper
        Pages 129-139
      3. Mario Mellado, Antonio Serrano, Carlos Mart’inez-A, José Miguel Rodríguez-Frade
        Pages 141-158
      4. Venkatakrishna R. Jala, Bodduluri Haribabu
        Pages 159-166
    3. Lipid Raft and Caveolae in Transmembrane Signaling

      1. Kathleen Boesze-Battaglia
        Pages 169-179
    4. Protein—Protein Interaction in Transmembrane Signaling

    5. Rho Guanosine Triphosphatases and Reconstitution of Signaling Complexes

      1. Mary Stofega, Celine DerMardirossian, Gary M. Bokoch
        Pages 269-279
      2. Shankar S. Iyer, David J. Kusner
        Pages 281-298
    6. Genomics and Proteomics

      1. George Lominadze, Richard A. Ward, Jon B. Klein, Kenneth R. McLeish
        Pages 343-356
  4. Back Matter
    Pages 357-364

About this book


The complete sequencing of the human genome and many new microarray and proteomic methods for analyzing global changes in both gene expression and protein profiles have greatly increased our ability to understand transmembrane signaling processes in close detail. In this fully updated and expanded second edition of Transmembrane Signaling Protocols, leading experts demonstrate the use of such techniques as single-cell imaging, bioluminescence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. Introductory chapters provide a wide-ranging perspective, including an overview of the many recent advances in our understanding of signal transduction pathways. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Transmembrane Signaling Protocols, Second Edition, makes available to novice and experienced researchers alike the readily reproducible techniques needed to advance the study of signal transduction pathways today.


Activation Proteomics Regulation Vivo gene expression genes membrane proteins molecular biology neutrophils regulation mechanisms saccharomyces cerevisiae signal transduction

Editors and affiliations

  • Hydar Ali
    • 1
  • Bodduluri Haribabu
    • 2
  1. 1.Department of Pathology, School of Dental MedicineThe University of PennsylvaniaPhiladelphia
  2. 2.Department of Microbiology and Immunology, James Graham Brown Cancer CenterUniversity of LouisvilleLouisville

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-546-0
  • Online ISBN 978-1-59745-048-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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