Embryonic Stem Cell Protocols

Volume 1: Isolation and Characterization

  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 329)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Isolation and Maintenance

    1. Front Matter
      Pages 1-1
    2. Yunhan Hong, Manfred Schartl
      Pages 3-16
    3. Hiroyuki Horiuchi, Shuichi Furusawa, Haruo Matsuda
      Pages 17-34
    4. Maren Schulze, Hendrik Ungefroren, Michael Bader, Fred Fändrich
      Pages 45-58
    5. Shigeo Saito, Ken Sawai, Arika Minamihashi, Hideyo Ugai, Takehide Murata, Kazunari K. Yokoyama
      Pages 59-79
    6. Hirofumi Suemori, Norio Nakatsuji
      Pages 81-89
  3. Signaling in Embryonic Stem Cell Differentiation

    1. Front Matter
      Pages 99-99
    2. Shaohua Li, Peter D. Yurchenco
      Pages 113-125
    3. Gabriella Minchiotti, Silvia Parisi, M. Graziella Persico
      Pages 151-170
    4. Sandy Becker, Laura Grabel
      Pages 171-185
    5. Sangmi Chung, Kwang-Soo Kim
      Pages 187-193
    6. Kenneth R. Boheler, Kirill V. Tarasov
      Pages 195-221
    7. Yoshimi Tokuzawa, Masayoshi Maruyama, Shinya Yamanaka
      Pages 223-231
    8. J. Matthew Velkey, Nicole A. Slawny, Theresa E. Gratsch, K. Sue O’shea
      Pages 233-261
  4. Genetic Manipulation of Embryonic Stem Cells

    1. Front Matter
      Pages 263-263
    2. Dieter Riethmacher, Filip Lim, Thomas Schimmang
      Pages 265-272
    3. Masahiro Oka, Lung-Ji Chang, Frank Costantini, Naohiro Terada
      Pages 273-281
    4. Katie M. Barrow, Flor M. Perez-Campo, Christopher M. Ward
      Pages 283-294
    5. Takayuki Asano, Hiroaki Shibata, Yutaka Hanazono
      Pages 295-303
    6. Tatsuyuki Takada, Yutaka Suzuki, Nae Kadota, Yasushi Kondo, Ryuzo Torii
      Pages 305-312
    7. Yiling Hong, Rachel B. Cervantes, Peter J. Stambrook
      Pages 313-326
    8. Dakang Xu, Trevor J. Wilson, Paul J. Hertzog
      Pages 327-338
  5. Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens

    1. Front Matter
      Pages 339-339
    2. Brigitte Wdziekonski, Phi Villageois, Cécile Vernochet, Blaine Phillips, Christian Dani
      Pages 341-351
    3. Patrick J. Mee, Carmel M. O’Brien, Hazel Thomson, Sjaak van der Sar, Viktor Lakics, Timothy E. Allsopp
      Pages 353-369
    4. Andrea E. M. Seiler, Roland Buesen, Anke Visan, Horst Spielmann
      Pages 371-395
    5. Sonja Becker, Martin Hrabé de Angelis, Johannes Beckers
      Pages 397-407
  6. Epigenetic Analysis of Embryonic Stem Cells

    1. Front Matter
      Pages 409-409
    2. Koichiro Nishino, Jun Ohgane, Masako Suzuki, Naka Hattori, Kunio Shiota
      Pages 421-445
  7. Tumor-Like Properties

    1. Front Matter
      Pages 447-447
    2. Kazutoshi Takahashi, Tomoko Ichisaka, Shinya Yamanaka
      Pages 449-458
    3. Takayuki Asano, Kyoko Sasaki, Yoshihiro Kitano, Keiji Terao, Yutaka Hanazono
      Pages 459-467
  8. Animal Models and Therapy

    1. Front Matter
      Pages 469-469
    2. Adeline A. Lau, Kim M. Hemsley, Adrian Meedeniya, Aaron J. Robinson, John J. Hopwood
      Pages 471-484
    3. Fumihiko Nishimura, Hayato Toriumi, Shigeaki Ishizaka, Toshisuke Sakaki, Masahide Yoshikawa
      Pages 485-493
  9. Back Matter
    Pages 495-510

About this book


Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.


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Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-498-2
  • Online ISBN 978-1-59745-037-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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