© 2006

Marijuana and Cannabinoid Research

Methods and Protocols

  • Emmanuel S. Onaivi

Part of the Methods In Molecular Medicine™ book series (MIMM, volume 123)

Table of contents

  1. Front Matter
    Pages i-xv
  2. George R. Uhl, Hiroki Ishiguro, Emmanuel S. Onaivi, Ping-Wu Zhang, B. Emmanuel Akinshola, Zhicheng Lin et al.
    Pages 1-17
  3. Nancy E. Buckley, Diep Burbridge, Manop Buranapramest, Tanya Ferguson, Renee Y. Paau
    Pages 19-40
  4. Patricia Tagliaferro, Alberto J. Ramos, Emmanuel S. Onaivi, Sergio G. Evrard, Maite Duhalde Vega, Alicia Brusco
    Pages 91-104
  5. Ganesh A. Thakur, Spyros P. Nikas, Richard I. Duclos Jr., Alexandros Makriyannis
    Pages 113-148
  6. Filomena Fezza, Natalia Battista, Monica Bari, Mauro Maccarrone
    Pages 163-168
  7. Adèle Thomas, Roger G. Pertwee
    Pages 191-207
  8. Stephen J. Heishman, Edward G. Singleton
    Pages 209-216
  9. David A. Gorelick, Stephen J. Heishman
    Pages 235-253
  10. Jean Lud Cadet, Karen Bolla, Ronald I. Herning
    Pages 255-268
  11. Ester Fride, Alex Perchuk, F. Scott Hall, George R. Uhl, Emmanuel S. Onaivi
    Pages 269-290
  12. Emmanuel S. Onaivi, Hiroki Ishiguro, Patel Sejal, Paul A. Meozzi, Lester Myers, Patricia Tagliaferro et al.
    Pages 291-298
  13. Back Matter
    Pages 299-306

About this book


The revolutionary discovery that the human body and brain make their own marijuana-like substances called endocannabinoids has transformed marijuana-cannabinoid research into a mainstream science impacting our understanding of reproduction, development, and cell death. In Marijuana and Cannabinoid Research: Methods and Protocols, prominent researchers and clinicians from around the world detail the cutting-edge in vitro and in vivo methods they are using to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. Other chapters describe methods to study the role of CBrs in immune function, the morphometric cytoskeletal components of neuronal and astroglial cells after cannabinoid treatment, and the in vitro and in vivo effects of cannabinoids in animals, including humans. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and electrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Marijuana and Cannabinoid Research: Methods and Protocols will help both basic and clinical investigators unravel the specific mechanisms of the marijuana-cannabinoid actions that are of central importance in biological and therapeutic research today.


cannabinoid clinical research depression marijuana membrane research

Editors and affiliations

  • Emmanuel S. Onaivi
    • 1
  1. 1.Department of BiologyWilliam Paterson UniversityWayne

Bibliographic information

  • Book Title Marijuana and Cannabinoid Research
  • Book Subtitle Methods and Protocols
  • Editors Emmanuel S. Onaivi
  • Series Title Methods In Molecular Medicine™
  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-350-3
  • Softcover ISBN 978-1-61737-513-2
  • eBook ISBN 978-1-59259-999-8
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages XVI, 306
  • Number of Illustrations 68 b/w illustrations, 0 illustrations in colour
  • Topics Pharmacy
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


"...will be a great asset internationally to emerging researchers embarking on investigations of cannabinoid seeking further understanding in their specific field, by expanding upon their exisiting methodologies to incorporate multi-disciplinary approaches." -Addiction