DNA Repair Protocols

Mammalian Systems

  • Daryl S. Henderson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 314)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, Thierry Magnaldo
    Pages 9-23
  3. William W. Au, Salama A. Salama
    Pages 25-42
  4. Shruti Nagar, James J. Corcoran, William F. Morgan
    Pages 43-50
  5. Nicolaas G. J. Jaspers, Malgorzata Z. Zdzienicka
    Pages 51-59
  6. Xuan Huang, Zbigniew Darzynkiewicz
    Pages 73-80
  7. George Iliakis, Nge Cheong
    Pages 95-108
  8. Michael J. Villalobos, Christopher J. Betti, Andrew T. M. Vaughan
    Pages 109-121
  9. George Iliakis, Bustanur Rosidi, Minli Wang, Huichen Wang
    Pages 123-131
  10. R. Michael Anson, Penelope A. Mason, Vilhelm A. Bohr
    Pages 155-181
  11. Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, Bennett Van Houten
    Pages 183-199
  12. Marcus S. Cooke, Alistair Robson
    Pages 215-228
  13. Betsy M. Sutherland, Paula V. Bennett, John C. Sutherland
    Pages 251-273

About this book

Introduction

In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair.

Keywords

DNA DNA replication Ligation PCR Polyacrylamid-Gelelektrophorese Quantitative PCR cell lines genes recombination reproducible techniques

Editors and affiliations

  • Daryl S. Henderson
    • 1
  1. 1.Pharmacological SciencesSUNY Stony BrookStony Brook

Bibliographic information

  • DOI https://doi.org/10.1385/1592599737
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-513-2
  • Online ISBN 978-1-59259-973-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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