Plant Cell Culture Protocols

  • Victor M. Loyola-Vargas
  • Felipe Vázquez-Flota

Part of the Methods in Molecular Biology™ book series (MIMB, volume 318)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Victor M. Loyola-Vargas, Felipe Vázquez-Flota
      Pages 3-8
    3. Trevor A. Thorpe
      Pages 9-32
  3. Cell Culture and Plant Regeneration: The Fundamentals

    1. Front Matter
      Pages 33-33
    2. Alan C. Cassells, Barbara M. Doyle
      Pages 35-50
    3. Gregorio Godoy-Hernández, Felipe A. Vázquez-Flota
      Pages 51-58
    4. Alejandrina Robledo-Paz, María Nélida Vázquez-Sánchez, Rosa María Adame-Alvarez, Alba Estela Jofre-Garfias
      Pages 59-70
    5. Lizbeth A. Castro-Concha, Rosa María Escobedo, María Lourdes de Miranda-Ham
      Pages 71-76
    6. Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten, Richard Visser
      Pages 101-109
    7. Francisco Quiroz-Figueroa, Miriam Monforte-González, Rosa M. Galaz-Ávalos, Victor M. Loyola-Vargas
      Pages 111-117

    1. Front Matter
      Pages 119-119
    2. Manuel L. Robert, José Luis Herrera-Herrera, Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo, Pedro Fuentes-Carrillo
      Pages 121-129
    3. Gabriela Fuentes, Carlos Talavera, Yves Desjardins, Jorge M. Santamaría
      Pages 131-144
    4. Michael E. Compton
      Pages 145-163
    5. Manuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, Miguel Angel Herrera-Alamillo
      Pages 165-178
    6. Zhihua Liao, Min Chen, Xiaofen Sun, Kexuan Tang
      Pages 179-185
    7. Trevor A. Thorpe, Indra S. Harry, Edward C. Yeung
      Pages 187-197
  5. Applications for Plant Protoplasts

    1. Front Matter
      Pages 199-199
    2. Michael R. Davey, Paul Anthony, J. Brian Power, Kenneth C. Lowe
      Pages 201-210
    3. Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch, Praveen K. Saxena
      Pages 211-217
    4. Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba, Nikolay Kuchuk
      Pages 219-232
    5. Gary Tallman
      Pages 233-252
    6. Juntaro Kato, Masahiro Mii
      Pages 253-262
  6. Protocols for Genomic Manipulation

    1. Front Matter
      Pages 263-263
    2. Indra K. Vasil, Vimla Vasil
      Pages 273-283
    3. Xiao-Mei Lu, Wei-Bo Yin, Zan-Min Hu
      Pages 285-303
    4. César De los Santos-Briones, Teresa Hernández-Sotomayor
      Pages 305-314
  7. Accumulation of Metabolites in Plant Cells

    1. Front Matter
      Pages 325-325
    2. Sang-Wook Park, Balakrishnan Prithiviraj, Ramarao Vepachedu, Jorge M. Vivanco
      Pages 335-347
    3. Elizabeta Hernández-Domínguez, Freddy Campos-Tamayo, Mildred Carrillo-Pech, Felipe Vázquez-Flota
      Pages 349-355
    4. Benjamin P. MacLeod, Peter J. Facchini
      Pages 357-368
  8. Back Matter
    Pages 369-394

About this book


Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites.
Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.


Agrobacterium tumefaciens Brassicaceae Chloroplast Embryo Flora Pathogen Protein biotechnology cell division cell expansion cryopreservation wheat

Editors and affiliations

  • Victor M. Loyola-Vargas
    • 1
  • Felipe Vázquez-Flota
    • 1
  1. 1.Unidad de Bioquímica y Biología Molecular de PlantasCentro de Investigacion Cientifica de YucatánYucatánMéxico

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-547-7
  • Online ISBN 978-1-59259-959-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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