© 2005

Calcium Signaling Protocols

  • David G. Lambert

Part of the Methods in Molecular Biology™ book series (MIMB, volume 312)

Table of contents

  1. Front Matter
    Pages i-x
  2. General

    1. Front Matter
      Pages 1-1
    2. Alec W. M. Simpson
      Pages 3-36
    3. Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, David G. Lambert
      Pages 37-45
  3. Specialist Measurement Systems

    1. Front Matter
      Pages 55-55
    2. Stephen C. Tovey, Paul J. Brighton, Gary B. Willars
      Pages 57-85
    3. Anthony J. Morgan, Andrew P. Thomas
      Pages 87-117
    4. Ian C. B. Marshall, Izzy Boyfield, Shaun McNulty
      Pages 119-124
    5. Ian C. B. Marshall, Davina E. Owen, Shaun McNulty
      Pages 125-131
  4. Measurement of Ca2+ Channel Activity

    1. Front Matter
      Pages 133-133
    2. Mercedes Villarroya, Manuela G. López, María F. Cano-Abad, Antonio G. García
      Pages 135-145
    3. Kazuyoshi Hirota, David G. Lambert
      Pages 147-159
    4. Atticus H. Hainsworth, Andrew D. Randall, Alessandro Stefani
      Pages 161-179
  5. Measurement of Ins(1,4,5,)P3 and Ca2+ Release From Intracellular Stores

About this book


Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition continues the excellence of the highly regarded first edition. New to this edition are chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for novice and expert researchers alike both theoretical and practical information for the optimal design and performance of studies of intracellular Ca2+ regulation.


ATP ATPase Calcium Endoplasmatisches Reticulum Nucleotide molecular biology protein reproducible techniques

Editors and affiliations

  • David G. Lambert
    • 1
  1. 1.Department of Cardiovascular Sciences, Division of Anaesthesia, Critical Care, and Pain ManagementLeicester Royal InfirmaryLeicesterUK

Bibliographic information

  • Book Title Calcium Signaling Protocols
  • Editors David G. Lambert
  • Series Title Methods in Molecular Biology™
  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-442-5
  • Softcover ISBN 978-1-61737-573-6
  • eBook ISBN 978-1-59259-949-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages X, 359
  • Number of Illustrations 61 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods


From Reviews of the First Edition...

"...will find a place on the bench in any lab that is moving into calcium signaling, or expanding its repertoire of techniques in calcium signaling. It will be valuable as a resource for...years to come." -Cell Biology International

"This book achieves much...For anyone remotely interested in Ca2+ it will be a very valuable resource in their lab" -Quarterly Review of Biology

". . .recommended to investigators interested in calcium measurement using optical methods. "-Doody's Health Sciences Book Review Journal

"Most protocols identify required materials such as buffers, reagents and cell lines. Methods are presented in a step-by-step format. Hopefully these protocols are adaptable by scientists for use in specific research. Certainly, the methods are specific enough not to leave the researcher stranded. Calcium Signaling Protocols is recommended for academic health and science libraries who support academic research."-E-Streams