© 2005

Antifungal Agents

Methods and Protocols

  • Erika J. Ernst
  • P. David Rogers

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 118)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Antifungal Resistance

    1. Front Matter
      Pages 13-13
    2. Shawn R. Lockhart, Claude Pujol, Andrew R. Dodgson, David R. Soll
      Pages 15-25
    3. Joachim Morschhäuser, Peter Staib, Gerwald Köhler
      Pages 27-34
    4. Joachim Morschhäuser, Peter Staib, Gerwald Köhler
      Pages 35-44
    5. Massoumeh Z. Hooshdaran, George M. Hilliard, P. David Rogers
      Pages 57-70
  4. Antifungal Development and Evaluation

    1. Front Matter
      Pages 81-81
    2. Melissa R. Jacob, Larry A. Walker
      Pages 83-109
    3. Russell E. Lewis, Nathan P. Wiederhold
      Pages 129-142
    4. Roxana G. Vitale, Javier Afeltra, Eric Dannaoui
      Pages 143-152
    5. Roxana G. Vitale
      Pages 153-158
  5. Host Responses and Immunotherapy

    1. Front Matter
      Pages 159-159
    2. Maria Simitsopoulou, Emmanuel Roilides
      Pages 161-179
    3. Floyd L. Wormley Jr., John R. Perfect
      Pages 193-198

About this book


With the growing frequency of fungal infections even as resistance to existing antifungal agents increases, methods for finding novel drug targets and evaluating antifungal resistance have become more important than ever. In Antifungal Agents: Methods and Protocols, expert scientists describe in detail the state-of-the-art molecular methods they have optimized for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. Many of the protocols were developed in the laboratory and, when a method is limited to specific organisms, include alternative techniques to expand their application. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists, and molecular biologists the productive tools they need today in order to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.


cytokine drug drug discovery fungal infection immunology immunotherapy infection infections molecular medicine resistance infectious diseases

Editors and affiliations

  • Erika J. Ernst
    • 1
  • P. David Rogers
    • 2
  1. 1.College of PharmacyUniversity of IowaIowa City
  2. 2.Departments of Pharmacy, Pharmaceutical Sciences, and Pediatrics, College of Pharmacy and MedicineUniversity of Tennessee Health Science CenterMemphis

Bibliographic information

  • Book Title Antifungal Agents
  • Book Subtitle Methods and Protocols
  • Editors Erika J. Ernst
  • Series Title Methods in Molecular Medicine™
  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-277-3
  • Softcover ISBN 978-1-61737-467-8
  • eBook ISBN 978-1-59259-943-1
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages X, 210
  • Number of Illustrations 30 b/w illustrations, 0 illustrations in colour
  • Topics Infectious Diseases
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Public Health
Internal Medicine & Dermatology


From the reviews:

"Due to the increased prevalence in systemic fungal infections worldwide, more knowledge is needed about antifungal drug testing and research is very important. This book, which is one in the series of ‘Methods in Molecular Medicine’, describes both traditional and new techniques for the investigation of antifungal resistance and for the development and evaluation of new compounds. … By and large, this is an interesting book for mycological researchers in both laboratory and clinical medicine." (Prof. Jan Faergemann, Acta Dermato-Venereologica, Vol. 86, August, 2006)