Protein-Ligand Interactions

Methods and Applications

  • G. Ulrich Nienhaus

Part of the Methods in Molecular Biology™ book series (MIMB, volume 305)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Edwin A. Lewis, Kenneth P. Murphy
    Pages 1-15
  3. Frank Gesellchen, Bastian Zimmermann, Friedrich W. Herberg
    Pages 17-45
  4. Jeffrey G. Mandell, Abel Baerga-Ortiz, Arnold M. Falick, Elizabeth A. Komives
    Pages 65-79
  5. Herbert P. Jennissen
    Pages 81-99
  6. Marius Schmidt, Hyotcherl Ihee, Reinhard Pahl, Vukica Šrajer
    Pages 115-154
  7. Till Maurer
    Pages 197-213
  8. Manho Lim, Philip A. Anfinrud
    Pages 243-259
  9. Uri Samuni, Joel M. Friedman
    Pages 287-300
  10. Mark S. Hargrove
    Pages 323-341
  11. Alison Rodger, Rachel Marrington, David Roper, Stuart Windsor
    Pages 343-363
  12. Lenka Zemanová, Andreas Schenk, Martin J. Valler, G. Ulrich Nienhaus, Ralf Heilker
    Pages 365-383
  13. Theodore L. Hazlett, Qiaoqiao Ruan, Sergey Y. Tetin
    Pages 415-438
  14. Sergio A. Hassan, Luis Gracia, Geetha Vasudevan, Peter J. Steinbach
    Pages 451-492
  15. Helmut Grubmüller
    Pages 493-515
  16. Back Matter
    Pages 555-568

About this book


Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions.


biology biosensors microscopy molecular biology protein proteins receptor

Editors and affiliations

  • G. Ulrich Nienhaus
    • 1
  1. 1.Department of BiophysicsUniversity of UlmUlmGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-372-5
  • Online ISBN 978-1-59259-912-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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