Overview
- Editors:
-
-
Tuofu Zhu
-
Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle
Access this book
Other ways to access
Table of contents (37 protocols)
-
Methods for Virus Isolation and Detection
-
- Susanne Polywka, Hedwig Duttmann, Frank Lübben, Rainer Laufs, Jürgen Feldner
Pages 229-243
-
-
-
- Anna Nordqvist, Eva Maria Fenyö
Pages 273-285
-
- Ann A. Kiessling, S. J. Eyre, B. D. Desmarais
Pages 287-313
-
Molecular Biology Methods
-
Front Matter
Pages 316-316
-
- Neeltje A. Kootstra, Hanneke Schuitemaker
Pages 317-325
-
- Hanneke Schuitemaker, Neeltje A. Kootstra
Pages 327-332
-
- Dalma Vödrös, Eva Maria Fenyö
Pages 333-342
-
- Philip J. Ellery, Suzanne M. Crowe
Pages 343-353
-
- Awet Abraha, Ryan M. Troyer, Miguel E. Quiñones-Mateu, Eric J. Arts
Pages 355-368
-
- Dawn M. Moore, Eric J. Arts, Yong Gao, Andre J. Marozsan
Pages 369-385
-
- Philip K. Ehrenberg, Nelson L. Michael
Pages 387-398
-
-
- Matthew Anderson, Patrick L. Green
Pages 409-421
-
- Roman Wirtz, Martin Löchelt
Pages 423-434
-
- Klaus-Ingmar Pfrepper, Rolf M. Flügel
Pages 435-444
-
- Stefano Aquaro, Carlo-Federico Perno
Pages 445-453
-
- Angélique B. van ’t Wout
Pages 455-469
-
About this book
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Reviews
"More than 20 years after the discoveries of HTLV and HIV, there appears to be a need for a consolidated reference of many of the standard and not-so-standard protocols used to characterize the human retroviruses. This book, containing some 37 detailed protocols provided by multinational authors in two parts, does an admirable job. Part I, therefore, includes guidance on isolation of HTLV, HIV and Human Foamy Virus (HFV) from peripheral blood, lymph nodes, CNS tissue and genital secretions (semen, vaginal fluids). It also describes a variety of molecular methods (largely PCR-based) to assess viral levels, integration status and drug sensitivity, plus quantification by antigen detection. At some 313 pages, Part I forms the substantial portion of the book. Part II (168 pages) guides on assessing cell and receptor tropism, construction and utilization of molecular clones and also use of microarrays to look at host and viral gene expression. The book on the whole is well illustrated with clear photographs and diagrams to support the detailed methodology, and the references at the end of each protocol are comprehensive. "-SGM Quarterly
Editors and Affiliations
-
Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle
Tuofu Zhu