Handbook of ELISPOT

Methods and Protocols

  • Alexander E. Kalyuzhny

Part of the Methods in Molecular Biology™ book series (MIMB, volume 302)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Elispot Assay Setup and Data Analysis

    1. Front Matter
      Pages 1-1
    2. Jonathon D. Sedgwick
      Pages 3-14
    3. Alexander E. Kalyuzhny
      Pages 15-31
    4. Sylvia Janetzki, Josephine H. Cox, Neal Oden, Guido Ferrari
      Pages 51-86
    5. Angela Grant, Sarah Palzer, Chris Hartnett, Tanya Bailey, Monica Tsang, Alexander E. Kalyuzhny
      Pages 87-94
    6. Carrie E. Peters, Steven M. Woodside, Allen C. Eaves
      Pages 95-115
  3. Elispot Assay in Animal Models

    1. Front Matter
      Pages 151-151
    2. Sushila K. Nordone, Rosemary Stevens, Alora S. LaVoy, Gregg A. Dean
      Pages 167-177
    3. Vanja Lazarevic, Santosh Pawar, JoAnne Flynn
      Pages 179-189
  4. Elispot Assays in Human Studies

    1. Front Matter
      Pages 205-205
    2. Jenna E. Ryan, Inna G. Ovsyannikova, Gregory A. Poland
      Pages 207-217
    3. Clara M. Pelfrey, Ioana R. Moldovan
      Pages 219-235
    4. Mark Matijevic, Robert G. Urban
      Pages 237-251
  5. Multiplex and Modified Elispot Assay Formats

    1. Front Matter
      Pages 261-261
    2. Yoshihiro Okamoto, Mikio Nishida
      Pages 263-272
    3. Sarah Palzer, Tanya Bailey, Chris Hartnett, Angela Grant, Monica Tsang, Alexander E. Kalyuzhny
      Pages 273-288
    4. Agnes Gazagne, Wolf Malkusch, Benoit Vingert, Wolf H. Fridman, Eric Tartour
      Pages 289-295
    5. Lisa A. Spencer, Rossana C. N. Melo, Sandra A. C. Perez, Peter F. Weller
      Pages 297-314
  6. Back Matter
    Pages 315-321

About this book


Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use.


Antigen Termination antibody biology cell chemistry cytokine cytokines development immune response interferon lymphocytes membrane reproducible techniques tuberculosis

Editors and affiliations

  • Alexander E. Kalyuzhny
    • 1
  1. 1.R&D Systems Inc.,Minneapolis

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-469-2
  • Online ISBN 978-1-59259-903-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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