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Ubiquitin-Proteasome Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Cam Patterson

    1. Department od Medicine and the Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill

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  2. Douglas M. Cyr

    1. Department of Cell and Developmental Biology, University of North Carolona at Chapel Hill, Chapel Hill

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 301)

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Table of contents (24 protocols)

  1. Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions

    1. Endoplasmic Reticulum-Associated Protein Quality Control and Degradation

      • Antje Schäfer, Dieter H. Wolf
      Pages 283-288

    2. Endoplasmic Reticulum-Associated Protein Quality Control and Degradation

      • Antje Schäfer, Dieter H. Wolf
      Pages 289-292

    3. Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells

      • J. Michael Younger, Chun-Yang Fan, Liling Chen, Meredith F. N. Rosser, Cam Patterson, Douglas M. Cyr
      Pages 293-303

    4. Aggresome Formation

      • Michael J. Corboy, Philip J. Thomas, W. Christian Wigley
      Pages 305-327

    5. Detection of Sumoylated Proteins

      • Roland S. Hilgarth, Kevin D. Sarge
      Pages 329-337

    6. Proteasome Inhibitors in Cancer Therapy

      • Robert Z. Orlowski
      Pages 339-350

    7. Parkinson’s Disease

      • Michael G. Schlossmacher, Hideki Shimura
      Pages 351-369

  2. Back Matter

    Pages 371-381
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Keywords

About this book

A collection of cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome-dependent functions. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.

Reviews

"...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field." - Doody's Health Science Book Review Journal

Editors and Affiliations

  • Department od Medicine and the Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill

    Cam Patterson

  • Department of Cell and Developmental Biology, University of North Carolona at Chapel Hill, Chapel Hill

    Douglas M. Cyr

Bibliographic Information

  • Book Title: Ubiquitin-Proteasome Protocols

  • Editors: Cam Patterson, Douglas M. Cyr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598951

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-252-0Published: 01 April 2005

  • Softcover ISBN: 978-1-61737-453-1Published: 10 November 2010

  • eBook ISBN: 978-1-59259-895-3Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 381

  • Number of Illustrations: 172 b/w illustrations

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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