Ubiquitin-Proteasome Protocols

  • Cam Patterson
  • Douglas M. Cyr

Part of the Methods in Molecular Biology™ book series (MIMB, volume 301)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Biochemical Methods to Study the Ubiquitin-Proteasome System

    1. Front Matter
      Pages 1-1
    2. Maria Gaczynska, Pawel A. Osmulski
      Pages 1-22
    3. Arthur L. Haas
      Pages 23-35
    4. Manabu Furukawa, Paul S. Andrews, Yue Xiong
      Pages 37-46
    5. Shahri Raasi, Cecile M. Pickart
      Pages 47-55
    6. Chang-Wei Liu, Elizabeth Strickland, George N. DeMartino, Philip J. Thomas
      Pages 71-81
    7. Stefan Tenzer, Hansjörg Schild
      Pages 97-115
    8. Carol E. Parker, Maria R. Warren, David R. Loiselle, Nedyalka N. Dicheva, Cameron O. Scarlett, Christoph H. Borchers
      Pages 117-151
    9. Carol E. Parker, Viorel Mocanu, Maria R. Warren, Susanna F. Greer, Christoph H. Borchers
      Pages 153-173
    10. Robert J. Lee, Ardythe A. McCracken, Jeffrey L. Brodsky
      Pages 175-184
    11. Eric Carlson, Nathan Bays, Larry David, William R. Skach
      Pages 185-205
    12. Nathaniel S. Russell, Keith D. Wilkinson
      Pages 207-219
  3. Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions

    1. Front Matter
      Pages 221-221
    2. Edward G. Mimnaugh, Leonard M. Neckers
      Pages 223-241
    3. R. Jürgen Dohmen, Markus K. London, Christoph Glanemann, Paula C. Ramos
      Pages 243-254
    4. Aaron Ciechanover
      Pages 255-270
    5. Shu-Bing Qian, Jack R. Bennink, Jonathan W. Yewdell
      Pages 271-281

About this book

Introduction

The targeted addition of ubiquitin to proteins is now known to regulate many cellular events, with the enzymes involved in protein ubiquitination linked to a variety of clinical problems, such as cancer, heart disease, and immune dysfunction. In Ubiquitin-Proteasome Protocols, hands-on leaders in the field, many of whom originally developed the methods they describe, detail cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome- dependent functions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.

Keywords

Endoplasmatisches Reticulum Termination biochemistry chemistry enzymes reproducible techniques

Editors and affiliations

  • Cam Patterson
    • 1
  • Douglas M. Cyr
    • 2
  1. 1.Department od Medicine and the Carolina Cardiovascular Biology CenterUniversity of North Carolina at Chapel HillChapel Hill
  2. 2.Department of Cell and Developmental BiologyUniversity of North Carolona at Chapel HillChapel Hill

Bibliographic information

  • DOI https://doi.org/10.1385/1592598951
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-252-0
  • Online ISBN 978-1-59259-895-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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