© 2005

Stroke Genomics

Methods and Reviews

  • Simon J. Read
  • David Virley

Part of the Methods in Molecular Medicine book series (MIMM, volume 104)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Simon J. Read, Frank C. Barone
      Pages 3-15
  3. Preclinical Models and Gene Manipulation

    1. Front Matter
      Pages 17-17
    2. Hilary V. O. Carswell, Martin W. McBride, Delyth Graham, Anna F. Dominiczak, I. Mhairi Macrae
      Pages 49-74
    3. Midori A. Yenari, Robert M. Sapolsky
      Pages 75-87
    4. Paul Stroemer, Helen Hodges
      Pages 89-104
    5. Frank C. Barone
      Pages 105-184
    6. Toby John Roberts
      Pages 203-220
  4. Clinical Paradigms

    1. Front Matter
      Pages 221-221
    2. Ahamad Hassan, Hugh S. Markus
      Pages 223-240
  5. Assessing Differential Expression

    1. Front Matter
      Pages 251-251
    2. Paul R. Nelson, Andrew B. Goulter, Richard J. Davis
      Pages 285-311

About this book


Although numerous genomic studies of stroke have examined such factors as stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery, much work to determine the precise role of gene function in human neurological pathophysiology remains ahead. In Stroke Genomics: Methods and Reviews, leading experts in the field explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. Providing the reader with cutting-edge reviews of clinical and preclinical genomics, the authors relate changes in gene expression to physiological end points-such as functional imaging paradigms-to produce a more holistic approach in which molecular biology goes hand in hand with stroke pathophysiology. Topics of special interest include stem cell transplantation, gene therapy, clinical gene/gene interaction studies, and cytokine drug discovery. Readily reproducible techniques are provided, along with ancillary methods (imaging, animal models, human genetic study design, and transgenic animal production) that can be effectively applied to gene discovery. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Stroke Genomics: Methods and Reviews shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke, work that will greatly advance its understanding, as well as rapid development of the next generation of stroke therapeutics.


Chromosom PCR gene expression gene therapy genes physiology transcription

Editors and affiliations

  • Simon J. Read
    • 1
  • David Virley
    • 2
  1. 1.AstraZeneca Pharmaceuticals MacclesfieldCheshireUK
  2. 2.Neurology Centre for Excellence in Drug Discovery GlaxoSmithKline PharmaceuticalsHarlowUK

Bibliographic information

  • Book Title Stroke Genomics
  • Book Subtitle Methods and Reviews
  • Editors Simon J. Read
    David Virley
  • Series Title Methods in Molecular Medicine
  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-333-6
  • Softcover ISBN 978-1-61737-500-2
  • eBook ISBN 978-1-59259-836-6
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 1
  • Number of Pages X, 351
  • Number of Illustrations 65 b/w illustrations, 0 illustrations in colour
  • Topics Neurosciences
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals


From the reviews:

"The protocols presented in Stroke Genomics: Methods and Reviews will greatly benefit scientists interested in the molecular biology as well as the genetic and genomic basis of strokes." - Journal of Chemical Neuroanatomy

"...very interesting and attractive and interesting overview of the different aspects of the current stroke research...can be recommended as a source of useful and up-to-date practical and theoretical information on stroke genetics and on stroke associated gene expression." - European Neurology

"This book gives the opportunity for experts in the field to explore the practical and experimental applications of genomic and molecular biology research to the treatment of stroke. … The book also indicates detailed methods for studying the molecular biology of stroke. … It can be recommended if one is interested in the basic scientific background that underlies central nervous system pathology … ." (J. V. Byrne, Neuroradiology, Vol. 47, 2005)