Epidermal Cells

Methods and Protocols

  • Kursad Turksen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 289)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Keratinocyte and Organ Cultures

    1. Front Matter
      Pages 1-1
    2. Annalisa Pirrone, Barbara Hager, Philip Fleckman
      Pages 3-14
    3. Richard P. Redvers, Pritinder Kaur
      Pages 15-22
    4. Monika Jost, Ulrich Rodeck
      Pages 23-28
    5. Nicole Maas-Szabowski, Norbert E. Fusenig, Hans-Jürgen Stark
      Pages 47-60
    6. Alexander Margulis, Weitian Zhang, Jonathan A. Garlick
      Pages 61-70
  3. Epidermal Stem Cells

    1. Front Matter
      Pages 71-71
    2. Wei-Yang Wu, Rebecca J. Morris
      Pages 73-77
    3. Amy Li, Pritinder Kaur
      Pages 87-96
    4. Danielle Larouche, Cindy Hayward, Kristine Cuffley, Lucie Germain
      Pages 103-110
  4. Analysis of Epidermal Differentiation

    1. Front Matter
      Pages 111-111
    2. Tammy-Claire Troy, Ramtin Rahbar, Bilge Diker, Kursad Turksen
      Pages 113-119
    3. Tammy-Claire Troy, Robert Man-Kit Cheung, Kursad Turksen
      Pages 121-126
    4. Ashley E. Webb, David Kimelman
      Pages 137-145
    5. Wing Y. Chang, Lina Dagnino
      Pages 147-156
    6. László G. Kömüves, Corey Largman
      Pages 157-169
    7. Kiyofumi Yamanishi, Chun-Shen Shen, Hitoshi Mizutani
      Pages 171-174
    8. Jörg Weiske, Otmar Huber
      Pages 175-191
    9. Maja Matic, Christopher Pullis, Marc G. Golightly, Sanford R. Simon
      Pages 193-199
    10. Takashi Kobayashi
      Pages 201-208
  5. Methods and Approaches for the Analysis for Epidermal Function

    1. Front Matter
      Pages 227-227
    2. Andrea Casasco, Antonia Icaro Cornaglia, Federica Riva, Marco Casasco, Alberto Calligaro
      Pages 229-238
    3. William R. Otto
      Pages 251-262
    4. Anthony M. Flores, Brian J. Aneskievich
      Pages 263-272
    5. Richard B. Presland, Philip Fleckman
      Pages 273-286
    6. Olga Igoucheva, Kyonggeun Yoon
      Pages 287-302
    7. David F. Fischer, Claude Backendorf
      Pages 303-313
    8. Sarah H. Williams, Alain Hovnanian
      Pages 315-328
    9. Daniel Metzger, Mei Li, Pierre Chambon
      Pages 329-340
    10. Jin-Jun Meng, Meghan Rojas, Willis Bacon, John T. Stickney, Wallace Ip
      Pages 341-357
    11. Jun Ohgane, Naka Hattori, Kunio Shiota
      Pages 371-382
    12. Bastiaan J. H. Jansen, Gys de Jongh, Joost Schalkwijk, Fred van Ruissen
      Pages 383-398
    13. Richard L. Davis Jr., Rusla M. DuBreuil, Shanker P. Reddy, Thomas P. Dooley
      Pages 399-412
  6. Transplantation and Gene Therapy

    1. Front Matter
      Pages 423-423
    2. Shari Greenberg, Alexander Margulis, Jonathan A. Garlick
      Pages 425-429
    3. Hiroki Maruyama, Jun-Ichi Miyazaki, Fumitake Gejyo
      Pages 431-436
    4. Robert M. Hoffman
      Pages 437-448
  7. Back Matter
    Pages 449-480

About this book


In recent years our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including wound healing and transplantation. In Epidermal Cells: Methods and Protocols, a panel of experienced basic and clinical researchers describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.


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Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-267-4
  • Online ISBN 978-1-59259-830-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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Health & Hospitals
Internal Medicine & Dermatology