Epigenetics Protocols

  • Trygve O. Tollefsbol

Part of the Methods in Molecular Biology™ book series (MIMB, volume 287)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Trygve O. Tollefsbol
    Pages 1-8
  3. Yan Yan, Haobin Chen, Max Costa
    Pages 9-19
  4. Alan W. Thorne, Fiona A. Myers, Tim R. Hebbes
    Pages 21-44
  5. Nicolas Steward, Hiroshi Sano
    Pages 65-75
  6. Qianjin Lu, Bruce Richardson
    Pages 77-86
  7. Cheng Liu, Dawei Xu
    Pages 87-97
  8. David Umlauf, Yuji Goto, Robert Feil
    Pages 99-120
  9. Joseph A. Baur, Woodring E. Wright, Jerry W. Shay
    Pages 121-136
  10. Eva Kirsten, Ernest Kun, Jerome Mendeleyev, Charles P. Ordahl
    Pages 137-149
  11. Mark D. Garfinkel, Vincent E. Sollars, Xiangyi Lu, Douglas M. Ruden
    Pages 151-168
  12. Liang Liu, Rebecca C. Wylie, Nathaniel J. Hansen, Lucy G. Andrews, Trygve O. Tollefsbol
    Pages 169-179
  13. Jean Benhattar, Geneviève Clément
    Pages 181-193
  14. Osman El-Maarri
    Pages 195-205
  15. Pearlly S. Yan, Susan H. Wei, Tim Hui-Ming Huang
    Pages 251-260
  16. Gerard J. Nuovo
    Pages 261-271
  17. Tomoki Yokochi, Keith D. Robertson
    Pages 285-296
  18. Back Matter
    Pages 297-302

About this book


Recent advances in epigenetic research as well as the development of exciting new technologies have helped greatly in unraveling the many mysteries of nontraditional genetic processes. In Epigenetics Protocols, hands-on researchers describe state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, Epigenetics Protocols offers investigators readily reproducible techniques that will further promote progress in this critically important field.

Editors and affiliations

  • Trygve O. Tollefsbol
    • 1
  1. 1.Department of BiologyUniversity of AlabamaBirmingham

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-336-7
  • Online ISBN 978-1-59259-828-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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