Apoptosis Methods and Protocols

  • Hugh J. M. Brady

Part of the Methods in Molecular Biology book series (MIMB, volume 282)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Demetrius Matassov, Terri Kagan, Julie Leblanc, Marianna Sikorska, Zahra Zakeri
    Pages 1-17
  3. Vicki Save, Peter A. Hall, Philip J. Coates
    Pages 67-84
  4. David J. McConkey, Leta Nutt
    Pages 117-130
  5. Carme Caelles, Mónica Morales
    Pages 145-156
  6. Jonathan Whitfield, Stephen J. Neame, Jonathan Ham
    Pages 157-168
  7. Stephen J. Neame, Jonathan Whitfield, Jonathan Ham
    Pages 169-178
  8. Ludmila Rizhsky, Vladimir Shulaev, Ron Mittler
    Pages 179-189
  9. Kimberly McCall, Jeanne S. Peterson
    Pages 191-205
  10. Andrew Devitt, Christopher D. Gregory
    Pages 207-221
  11. Karen Heyninck, Sofie Van Huffel, Marja Kreike, Rudi Beyaert
    Pages 223-241
  12. Wim van Criekinge, Peter Schotte, Karen Heyninck, Rudi Beyaert
    Pages 243-254
  13. Michael Hubank, Fredrik Bryntesson, Jennifer Regan, David G. Schatz
    Pages 255-273
  14. Joëlle Thomas, Yann Leverrier, Anne-Laure Mathieu, Jacqueline Marvel
    Pages 275-289
  15. Back Matter
    Pages 291-304

About this book


The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.

Editors and affiliations

  • Hugh J. M. Brady
    • 1
  1. 1.Institute of Child HealthUniversity College LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-873-8
  • Online ISBN 978-1-59259-812-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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