Protein NMR Techniques

  • Downing A. Kristina 

Part of the Methods in Molecular Biology™ book series (MIMB, volume 278)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Lorraine Hewitt, James M. McDonnell
    Pages 1-16
  3. David Cowburn, Alexander Shekhtman, Rong Xu, Jennifer J. Ottesen, Tom W. Muir
    Pages 47-56
  4. Guang Zhu, Youlin Xia, Donghai Lin, Xiaolian Gao
    Pages 57-78
  5. Kieran Fleming, Stephen Matthews
    Pages 79-88
  6. Eva de Alba, Nico Tjandra
    Pages 89-106
  7. Christian Griesinger, Wolfgang Peti, Jens Meiler, Rafael Brüschweiler
    Pages 107-122
  8. Luke M. Rooney, Sachchidanand, Jörn M. Werner
    Pages 123-138
  9. Guang Zhu, Youlin Xia, Donghai Lin, Xiaolian Gao
    Pages 161-184
  10. James G. Kempf, J. Patrick Loria
    Pages 185-231
  11. Daniel Nietlispach, Helen R. Mott, Katherine M. Stott, Peter R. Nielsen, Abarna Thiru, Ernest D. Laue
    Pages 255-288
  12. Gabriele Varani, Yu Chen, Thomas C. Leeper
    Pages 289-312
  13. Peter Güntert
    Pages 353-378
  14. Michael Habeck, Wolfgang Rieping, Jens P. Linge, Michael Nilges
    Pages 379-402
  15. Anthony Watts, Suzana K. Straus, Stephan L. Grage, Miya Kamihira, Yuen Han Lam, Xin Zhao
    Pages 403-473
  16. Back Matter
    Pages 475-487

About this book


Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology™ series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules.

Editors and affiliations

  • Downing A. Kristina 
    • 1
  1. 1.Department of BiochemistryUniversity of OxfordOxfordUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-246-9
  • Online ISBN 978-1-59259-809-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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