Photosynthesis Research Protocols

1. Front Matter

   Pages i-xiii

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2. Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids

   • Juan Cuello, MarÍa José Quiles

   Pages 1-9

3. Isolation of Photosystem I Particles From Spinach

   • Tetsuo Hiyama

   Pages 11-17

4. Rapid Isolation and Purification of Photosystem I Chlorophyll-Binding Protein From Chlamydomonas reinhardtii

   • Velupillai M. Ramesh, Andrew N. Webber

   Pages 19-28

5. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants

   • Yasusi Yamamoto, Shinsuke Sakuma, Jian-Ren Shen

   Pages 29-36

6. Isolation of Functional Photosystem II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803

   • Dmitrii V. Vavilin

   Pages 37-47

7. Isolation of Photosystem I Reaction Center Preparation From Spinach

   • Tetsuo Hiyama

   Pages 49-51

8. Isolation of Photosystem II Reaction Center Complexes From Plants

   • Michael Seibert, Inmaculada Yruela, Rafael Picorel

   Pages 53-62

9. Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach

   • Tetsuo Hiyama

   Pages 63-66

10. Purification and Crystallization of the Cytochrome b ₆ f Complex in Oxygenic Photosynthesis

    • Huamin Zhang, William A. Cramer

    Pages 67-78

11. Purification of Plastocyanin and Cytochrome c ₆ From Plants, Green Algae, and Cyanobacteria

    • José A. Navarro, Manuel Hervás, Miguel A. De la Rosa

    Pages 79-92

12. Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex

    • Wolfgang Rühle, Harald Paulsen

    Pages 93-103

13. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions

    • Ilian Simidjiev, Zsuzsanna Várkonyi, Gyözö Garab

    Pages 105-114

14. Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II

    • Grzegorz Jackowski

    Pages 115-128

15. Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants

    • Rafael Picorel, Miguel Alfonso, Michael Seibert

    Pages 129-135

16. The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Spectrophotometry

    • Tessa Pocock, Marianna Król, Norman P. A. Huner

    Pages 137-148

17. Isolation and Identification of Chloroplast Lipids

    • Norihiro Sato, Mikio Tsuzuki

    Pages 149-157

18. DNA Adducts With Chlorophyll and Chlorophyllin As Antimutagenic Agents

    • Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, Stavroula Diamantoglou

    Pages 159-171

19. Incorporation and Analysis of LHCII in Model Systems

    • Wieslaw I. Gruszecki

    Pages 173-181

20. Photosystem II Reconstitution Into Proteoliposomes

    • Mário Fragata

    Pages 183-204

Photosynthesis is one of the most important biological phenomena on earth. The conversion of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Photosynthesis is not only at the heart of plant bioenergetics, it is also fundamental to plant prod- tivity and biomass. Photosynthetic carbon fixation and oxygen evolution - rectly intervene in many environmental, including the global atmospheric CO 2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Several biochemical methods of isolation, treatment, and analysis have been developed to fulfill the needs of photosynthesis research. Photosynthesis Research Protocols contains a broad range of general and fundamental me- ods that are commonly used by plant biochemists, physiologists, and mole- lar biologists. This book is thus intended as a source of information for scientists working on any of the multiple aspects of photosynthesis, and should be of great interest to a multidisciplinary field of research involving agric- ture, biochemistry, biotechnology, botany, cell biology, environmental s- ences, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, plant physiology, plant stress, etc.

From the reviews:

"This book series is well known as a competent tool for research protocols in all fields of life science. … The book addresses either beginners in photosynthesis research or experienced researchers who want to extend their methodological experience. … I can recommend the book as basic literature for advanced courses in photosynthesis. … In conclusion, this is an excellent book which can be recommended for all laboratories involved in photosynthesis education and research." (Christian Wilhelm, Journal of Plant Physiology, Vol. 162 (6), 2005)

• Départment de Chimie-Biologie (GREIB), Université du Québec à Trois-Rivières, Trois-Rivières, Canada

  Robert Carpentier

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