Vaccinia Virus and Poxvirology

Methods and Protocols

  • Stuart N. Isaacs

Part of the Methods in Molecular Biology book series (MIMB, volume 269)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Stuart N. Isaacs
    Pages 1-13
  3. Maríia M. Lorenzo, Inmaculada Galindo, Rafael Blasco
    Pages 15-30
  4. Chelsea M. Byrd, Dennis E. Hruby
    Pages 31-40
  5. Mohamed Ragaa Mohamed, Edward G. Niles
    Pages 41-50
  6. Ernest S. Smith, Shuying Shi, Maurice Zauderer
    Pages 65-75
  7. Caroline Staib, Ingo Drexler, Gerd Sutter
    Pages 77-99
  8. Girish J. Kotwal, Melissa-Rose Abrahams
    Pages 101-112
  9. Hermann Meyer, Inger K. Damon, Joseph J. Esposito
    Pages 119-133
  10. Steven S. Broyles, Marcia Kremer
    Pages 135-142
  11. Paul D. Gershon
    Pages 151-167
  12. Paula Traktman, Kathleen Boyle
    Pages 169-185
  13. Brian M. Ward
    Pages 205-218
  14. Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, Miles W. Carroll
    Pages 243-265
  15. Nia Tatsis, Gomathinayagam Sinnathamby, Laurence C. Eisenlohr
    Pages 267-287
  16. Jill Schriewer, R. Mark L. Buller, Gelita Owens
    Pages 289-307
  17. Katharine N. Bossart, Christopher C. Broder
    Pages 309-331
  18. Chris Upton
    Pages 347-370
  19. Back Matter
    Pages 385-396

About this book


Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols, internationally recognized experts and leaders in poxvirology describe in step-by-step detail the successful techniques they have perfected to study and work with vaccinia virus and other poxviruses. They offer cutting-edge techniques for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxvirus-the latter critical to the important role of vaccinia virus in smallpox vaccination and its potential role as a vaccine vector directed against infectious agents and cancer. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and timely, Vaccinia Virus and Poxvirology: Methods and Protocols offers virologists and vaccinologists an unequaled source of proven techniques for understanding poxviruses and developing defenses against their use as bioterrorist agents.

Editors and affiliations

  • Stuart N. Isaacs
    • 1
  1. 1.Division of Infectious DiseasesUniversity of PennsylvaniaPhiladelphia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-229-2
  • Online ISBN 978-1-59259-789-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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