Flow Cytometry Protocols

  • Teresa S. Hawley
  • Robert G. Hawley

Part of the Methods in Molecular Biology™ book series (MIMB, volume 263)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Alice L. Givan
    Pages 1-31
  3. Howard M. Shapiro, Gerhard Nebe-von-Caron
    Pages 33-43
  4. Carleton C. Stewart, Sigrid J. Stewart
    Pages 45-65
  5. Omar D. Perez, Peter O. Krutzik, Garry P. Nolan
    Pages 67-94
  6. Holden T. Maecker
    Pages 95-107
  7. Alice L. Givan, Jan L. Fisher, Mary G. Waugh, Nadège Bercovici, Paul K. Wallace
    Pages 109-124
  8. Luzheng Liu, Beverly Z. Packard, Martin J. Brown, Akira Komoriya, Mark B. Feinberg
    Pages 125-139
  9. William G. Telford, Akira Komoriya, Beverly Z. Packard
    Pages 141-159
  10. Shannon S. Eaker, Teresa S. Hawley, Ali Ramezani, Robert G. Hawley
    Pages 161-180
  11. Teresa S. Hawley, Donald J. Herbert, Shannon S. Eaker, Robert G. Hawley
    Pages 219-237
  12. Steven C. Pruitt, Lawrence M. Mielnicki, Carleton C. Stewart
    Pages 239-258
  13. David W. Galbraith, Rangasamy Elumalai, Fang Cheng Gong
    Pages 259-279
  14. Francis Ka-Ming Chan, Kevin L. Holmes
    Pages 281-292
  15. Sam Lievens, José Van der Heyden, Els Vertenten, Jean Plum, Joël Vandekerckhove, Jan Tavernier
    Pages 293-309
  16. Michael Feldhaus, Robert Siegel
    Pages 311-332
  17. Marielle Cavrois, Jason Neidleman, Martin Bigos, Warner C. Greene
    Pages 333-343
  18. Michael G. Ormerod
    Pages 345-354
  19. Adel K. El-Naggar, Philippe Vielh
    Pages 355-370
  20. Adel K. El-Naggar
    Pages 371-384
  21. William G. Telford
    Pages 399-418
  22. Stephen P. Perfetto, David R. Ambrozak, Mario Roederer, Richard A. Koup
    Pages 419-424
  23. Back Matter
    Pages 425-434

About this book


In this thoroughly revised and updated edition of a widely used practical guide to flow cytometry, leading experimentalists describe in step-by-step detail an array of time-proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the fields of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high-throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T-cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Flow Cytometry Protocols, Second Edition offers both basic and clinical scientists an up-to-date collection of cutting-edge protocols for solving the widest variety of novel flow cytometry problems.

Editors and affiliations

  • Teresa S. Hawley
    • 1
  • Robert G. Hawley
    • 1
    • 2
  1. 1.American Red CrossRockville
  2. 2.The George Washington UniversityWashington, DC

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-234-6
  • Online ISBN 978-1-59259-773-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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