Molecular Diagnosis of Cancer

Methods and Protocols

  • Joseph E. Roulston
  • John M. S. Bartlett

Part of the Methods in Molecular Medicine book series (MIMM, volume 97)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael Scott, Peter A. Hall
    Pages 1-11
  3. Joseph E. Roulston
    Pages 13-27
  4. Anthony Rhodes, Diana M. Barnes
    Pages 29-57
  5. John M. S. Bartlett, Helen Speirs
    Pages 59-70
  6. Joanne Edwards, James J. Going, John M. S. Bartlett
    Pages 71-75
  7. John M. S. Bartlett
    Pages 77-87
  8. John M. S. Bartlett, Amanda Forsyth
    Pages 89-101
  9. Mark W. Drummond, Elaine K. Allan, Andrew Pearce, Tessa L. Holyoake
    Pages 103-116
  10. Lukas Bubendorf, Bruno Grilli
    Pages 117-128
  11. Jorma Isola, Minna Tanner
    Pages 133-144
  12. Linda M. Wasserman
    Pages 181-189
  13. Jenni Hakkarainen, Judith A. Welsh, Kirsi H. Vähäkangas
    Pages 191-208
  14. Kwan-Chee Allen Chan, Yuk-Ming Dennis Lo
    Pages 217-236
  15. Gillian Gifford, Robert Brown
    Pages 237-250
  16. Jeremy M. E. Taylor, Peter H. Kay, Dominic V. Spagnolo
    Pages 251-265
  17. Maiko Matsushita, Rie Yamazaki, Yutaka Kawakami
    Pages 267-275
  18. W. Nicol Keith, Aileen J. Monaghan
    Pages 311-322
  19. Evelyne Lopez-Crapez, Thierry Livache, Patrice Caillat, Daniela Zsoldos
    Pages 337-354
  20. Ekaterina Pestova, Kim Wilber, Walter King
    Pages 355-375
  21. Ronald Simon, Martina Mirlacher, Guido Sauter
    Pages 377-389
  22. Back Matter
    Pages 391-393

About this book


The growing tide of novel therapeutics that target molecules within a specific individual's cancer cells-over 300 such agents are currently in phase I or II clinical trials-makes it essential to employ appropriate diagnostic methods and approaches when selecting patients for treatment. In Molecular Diagnosis of Cancer: Methods and Protocols, a panel of leading international experts in molecular diagnostic medicine present a diverse collection of readily reproducible methods, derived from their own research for cancer detection. Highlights include FISH-based methodologies currently used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies-including sequence-specific oligonucleotide arrays and CGH arrays-and methodologies directed at the detection of epigenetic events and at quantitative gene expression. The authors apply these novel diagnostic procedures to a broad range of cases taken from cytology, solid tumor pathology, hematology, and rare cell detection, paying special attention to potential future developments and the practical problems of dealing with quality control and accuracy. The protocols presented follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Molecular Diagnosis of Cancer: Methods and Protocols offers pathologists providing molecular diagnostic services and researchers seeking molecularly targeted therapeutic approaches a broad array of today's most powerful methods and techniques.

Editors and affiliations

  • Joseph E. Roulston
    • 1
  • John M. S. Bartlett
    • 2
  1. 1.Division of Reproductive and Developmental SciencesThe University of Edinburgh, The Royal InfirmaryEdinburghScotland, UK
  2. 2.Division of Cancer and Molecular Pathology, University Department of SurgeryGlasgow Royal InfirmaryGlasgowScotland, UK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-160-8
  • Online ISBN 978-1-59259-760-4
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • Buy this book on publisher's site
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