Bacterial Artificial Chromosomes

Volume 2 Functional Studies

  • Shaying Zhao
  • Marvin Stodolsky

Part of the Methods in Molecular Biology book series (MIMB, volume 256)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Michael M. Weil, Rashmi Pershad, Ruoping Wang, Sheng Zhao
    Pages 1-6
  3. Scott E. Wenderfer, John J. Monaco
    Pages 7-20
  4. Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Daniel Pinkel, Donna G. Albertson
    Pages 39-56
  5. Sangdun Choi
    Pages 57-67
  6. Zunde Wang, Angelika Longacre, Peter Engler
    Pages 69-76
  7. Srividya Swaminathan, Shyam K. Sharan
    Pages 89-106
  8. Joep P. P. Muyrers, Youming Zhang, Vladmir Benes, Giuseppe Testa, Jeanette M. J. Rientjes, A. Francis Stewart
    Pages 107-121
  9. Giuseppe Testa, Kristina Vintersten, Youming Zhang, Vladmir Benes, Joep P. P. Muyrers, A. Francis Stewart
    Pages 123-139
  10. Kristina Vintersten, Giuseppe Testa, A. Francis Stewart
    Pages 141-158
  11. Vikki M. Marshall, Janette Allison, Tanya Templeton, Simon J. Foote
    Pages 159-182
  12. Deborah A. Swing, Shyam K. Sharan
    Pages 183-198
  13. Mathias Ackermann
    Pages 199-219
  14. Eva-Maria Borst, Irena Crnkovic-Mertens, Martin Messerle
    Pages 221-239
  15. Thomas G. H. Heister, Andrea Vögtlin, Lars Müller, Irma Heid, Cornel Fraefel
    Pages 241-256
  16. Markus Wagner, Ulrich H. Koszinowski
    Pages 257-268
  17. Eva-Maria Borst, György Pósfai, Frank Pogoda, Martin Messerle
    Pages 269-279
  18. Alistair McGregor, Mark R. Schleiss
    Pages 281-302
  19. Back Matter
    Pages 309-323

About this book


The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 2: Functional Studies provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 1: Library Construction, Physical Mapping, and Sequencing, presents readily reproducible techniques for library construction, physical mapping, and sequencing.
Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.

Editors and affiliations

  • Shaying Zhao
    • 1
  • Marvin Stodolsky
    • 2
  1. 1.The Institute for Genomic ResearchRockville
  2. 2.Office of Biological Environmental ResearchUS Department of EnergyGermantown

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-989-6
  • Online ISBN 978-1-59259-753-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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