Bacterial Artificial Chromosomes

Volume 1 Library Construction, Physical Mapping, and Sequencing

  • Shaying Zhao
  • Marvin Stodolsky

Part of the Methods in Molecular Biology™ book series (MIMB, volume 255)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Kazutoyo Osoegawa, Pieter J. de Jong
    Pages 1-46
  3. David Šmajs, Steven J. Norris, George M. Weinstock
    Pages 47-56
  4. Sangita Pal, Solida Mak, George M. Weinstock
    Pages 57-68
  5. Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, Vladimir Larionov
    Pages 69-89
  6. Tim S. Poulsen
    Pages 91-100
  7. Chang-Su Lim, Ung-Jin Kim
    Pages 101-111
  8. Heike Zimdahl, Claudia Gösele, Thomas Kreitler, Margit Knoblauch
    Pages 113-129
  9. Xiao-Ning Chen, Julie R. Korenberg
    Pages 131-141
  10. Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, Marco Marra
    Pages 143-156
  11. Tim S. Poulsen, Hans E. Johnsen
    Pages 157-161
  12. Michael Olivier, Shannon Brady, David R. Cox
    Pages 163-169
  13. Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina et al.
    Pages 189-196
  14. Rachel Reeg, Anup Madan
    Pages 197-210
  15. Elahe Elahi, Mostafa Ronaghi
    Pages 211-219
  16. Christine Bird, Darren Grafham
    Pages 255-277
  17. Mihai Pop, Dan Kosack
    Pages 279-294
  18. Andrei Malykh, Olga Malykh, Nikolai Polushin, Sergei Kozyavkin, Alexei Slesarev
    Pages 295-308
  19. Diana Radune, Hervé Tettelin
    Pages 309-318
  20. Jeremy Schmutz, Jane Grimwood, Richard M. Myers
    Pages 319-332
  21. Jeremy Schmutz, Jane Grimwood, Richard M. Myers
    Pages 333-342
  22. Jeremy Schmutz, Jane Grimwood, Richard M. Myers
    Pages 343-349
  23. Back Matter
    Pages 351-360

About this book


The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms.
Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.


BAC Chromosom DNA DNA sequencing PAC hybridization reproducible techniques

Editors and affiliations

  • Shaying Zhao
    • 1
  • Marvin Stodolsky
    • 2
  1. 1.The Institute for Genomic ResearchRockville
  2. 2.Office of Biological and Environmental ResearchUS Department of EnergyGermantown

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-988-9
  • Online ISBN 978-1-59259-752-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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