Ribozymes and siRNA Protocols

  • Mouldy Sioud

Part of the Methods in Molecular Biology™ book series (MIMB, volume 252)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Rajesh K. Gaur, Andreas Hanne, Guido Krupp
    Pages 9-17
  3. Kumud K. Singh, Andreas Hanne, Guido Krupp
    Pages 33-48
  4. Dagmar Klein, Camillo Ricordi, Ricardo L. Pastori
    Pages 49-56
  5. Soumitra Basu, Catherine Pazsint, Gopeswar Chowdhury
    Pages 57-75
  6. Hsiu-Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi, Arthur D. Riggs
    Pages 109-123
  7. Wei-Hua Pan, Gary A. Clawson
    Pages 125-144
  8. Adam Roth, Ronald R. Breaker
    Pages 145-164
  9. Yasuo Komatsu, Eiko Ohtsuka
    Pages 165-177
  10. Emma T. Bowden, Anna T. Riegel
    Pages 179-194
  11. Masayuki Sano, Kazunari Taira
    Pages 195-207
  12. Roger Abounader, Robert Montgomery, Harry Dietz, John Laterra
    Pages 209-219
  13. Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin, William W. Hauswirth
    Pages 221-236
  14. Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara, Kazunari Taira
    Pages 237-243
  15. Yoshio Kato, Masaru Tsunemi, Makoto Miyagishi, Hiroaki Kawasaki, Kazunari Taira
    Pages 245-256
  16. Mayu Iyo, Hiroaki Kawasaki, Kazunari Taira
    Pages 257-265
  17. Murray J. Cairns, Lun-Quan Sun
    Pages 267-277
  18. Samitabh Chakraborti, Bandi Sriram, Akhil C. Banerjea
    Pages 279-290
  19. Murray J. Cairns, Lun-Quan Sun
    Pages 291-302
  20. Peter B. Rupert, Adrian R. Ferré-D’Amaré
    Pages 303-311
  21. Alicia Barroso-delJesus, Elena Puerta-Fernández, Cristina Romero-López, Alfredo Berzal-Herranz
    Pages 313-325
  22. Cristina Romero-López, Alicia Barroso-delJesus, Elena Puerta-Fernñndez, Alfredo Berzal-Herranz
    Pages 327-338
  23. Max W. Richardson, Linda Hostalek, Michelle Dobson, Jason Hu, Richard Shippy, Andrew Siwkowski et al.
    Pages 339-358
  24. Christer Einvik, Tonje Fiskaa, Eirik W. Lundblad, Steinar Johansen
    Pages 359-371
  25. Hua Zou, Karen Chan, Phong Trang, Fenyong Liu
    Pages 385-398
  26. Mouldy Sioud, Marianne Leirdal
    Pages 457-469
  27. Dun Yang, Andrei Goga, J. Michael Bishop
    Pages 471-482
  28. Makoto Miyagishi, Kazunari Taira
    Pages 483-491
  29. Hidetoshi Hasuwa, Masaru Okabe
    Pages 501-508
  30. Mouldy Sioud, Dag R. Sørensen
    Pages 515-522
  31. Changxian Shen, Sven N. Reske
    Pages 523-532
  32. Hideo Akashi, Makoto Miyagishi, Kazunari Taira
    Pages 533-543
  33. Olivier Seksek, Jacques Bolard
    Pages 545-568
  34. Frances K. Ngok, Ronald T. Mitsuyasu, Janet L. Macpherson, Maureen P. Boyd, Geoff P. Symonds, Rafael G. Amado
    Pages 581-598
  35. Maureen P. Boyd, Frances K. Ngok, Alison V. Todd, Geoff P. Symonds, Janet L. Macpherson, Rafael G. Amado
    Pages 599-616
  36. Back Matter
    Pages 617-623

About this book


In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, hammerhead ribozymes and derivatives, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, RNase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozyme protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequence-specific gene silencing in a wide variety of organisms. Additional techniques cover the analysis of ribozyme structures and conformational transitions using nucleotide analog interference mapping and fluorescence resonance energy transfer, the use of ribozymes in clinical and gene therapy, and the use of ribozymes and DNAzymes in rodent models of human disease. Each proven protocol includes a background introduction outlining the principle behind the technique, step-by-step instructions, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Ribozymes and siRNA Protocols details for experienced and novice investigators alike the many exciting advances in our understanding of nucleic acid enzymes, as well as demonstrating how they may be used to analyze gene function and target validation, and to productively develop novel therapeutics for human diseases.

Editors and affiliations

  • Mouldy Sioud
    • 1
  1. 1.Institute of Cancer ResearchThe Norwegian Radium HospitalOsloNorway

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-226-1
  • Online ISBN 978-1-59259-746-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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