© 2004

HPLC of Peptides and Proteins

Methods and Protocols

  • Marie-Isabel Aguilar

Part of the Methods in Molecular Biology™ book series (MIMB, volume 251)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Modes of HPLC

    1. Front Matter
      Pages 1-1
    2. Marie-Isabel Aguilar
      Pages 3-8
    3. Peter Stanton
      Pages 23-43
    4. Peter Stanton
      Pages 55-73
    5. Herbert Lindner, Wilfried Helliger
      Pages 75-88
    6. Stuart R. Gallant
      Pages 103-109
  3. Preparative Methodologies

    1. Front Matter
      Pages 141-141
    2. Montserrat Carrascal, Joaquin Abian
      Pages 143-164
    3. Edouard C. Nice, Marie-Isabel Aguilar
      Pages 165-175
    4. Edouard C. Nice, Marie-Isabel Aguilar
      Pages 177-182
    5. Kálmán Benedek
      Pages 183-190
    6. Denis B. Scanlon, James Finlayson
      Pages 191-209
    7. Joseph Bertolini, Peter Gomme, Patrick Thomas
      Pages 211-223
  4. Applications

    1. Front Matter
      Pages 225-225
    2. Peter Højrup
      Pages 227-244

About this book


High performance liquid chromatography (HPLC) plays a critical role today in both our understanding of biological processes and in the development of peptide and protein-based pharmaceuticals. In HPLC of Peptides and Proteins: Methods and Protocols, leading experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. These applications include proteolytic mapping, posttranslational modification, neuropeptide processing, glycopeptides and glycoproteins, MHC-binding peptides, toxins/venoms, membrane proteins, antibodies, combinatorial and proteome analysis, and enzymatic activity. Each readily reproducible protocol contains background notes, step-by-step instructions, reagent and equipment lists, and tips on both troubleshooting and avoiding known pitfalls.
Comprehensive and easy-to-follow, HPLC of Peptides and Proteins: Methods and Protocols offers every biologist and biotechnologist at all levels of expertise ready access to the powerful tools needed to develop and carry out peptide and protein separations and analyses today.

Editors and affiliations

  • Marie-Isabel Aguilar
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyMonash UniversityClaytonAustralia

Bibliographic information

  • Book Title HPLC of Peptides and Proteins
  • Book Subtitle Methods and Protocols
  • Editors Marie-Isabel Aguilar
  • Series Title Methods in Molecular Biology™
  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-0-89603-977-3
  • Softcover ISBN 978-1-61737-286-5
  • eBook ISBN 978-1-59259-742-0
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 414
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
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"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical protocols...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics

"...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography