Developmental Biology Protocols

Volume I

  • John M. Walker
  • Rocky S. Tuan
  • Cecilia W. Lo

Part of the Methods in Molecular Biology™ book series (MIMB, volume 135)

Table of contents

  1. Front Matter
    Pages i-xxv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Rocky S. Tuan, Cecilia W. Lo
      Pages 3-5
  3. Systems: Production, Culture, and Storage

    1. Front Matter
      Pages 7-7
    2. Christopher J. Lowe, Gregory A. Wray
      Pages 9-15
    3. Diana K. Darnell, Gary C. Schoenwolf
      Pages 25-29
    4. Diana K. Darnell, Gary C. Schoenwolf
      Pages 31-38
    5. Tamao Ono
      Pages 39-46
    6. Adam S. Doherty, Richard M. Schultz
      Pages 47-52
    7. Jean Richa
      Pages 77-82
  4. Developmental Pattern and Morphogenesis

    1. Front Matter
      Pages 83-83
    2. Robert Finkelstein
      Pages 85-88
    3. Takashi Ariizumi, Kazuhiro Takano, Makoto Asashima, George M. Malacinski
      Pages 89-112
    4. Gary C. Schoenwolf, Jodi L. Smith
      Pages 113-125
    5. Brian K. Hall, Tom Miyake
      Pages 127-137
    6. Michael G. Narotsky, John M. Rogers
      Pages 139-150
    7. B. Rush Waller III, Andy Wessels
      Pages 151-161
  5. Embryo Structure and Function

    1. Front Matter
      Pages 163-163
    2. Charles D. Little, Christopher J. Drake
      Pages 183-189
    3. Robert M. Zucker, E. Sidney Hunter III, John M. Rogers
      Pages 191-202
    4. Bradley R. Smith
      Pages 211-216
    5. Stephen A. Boppart, Mark E. Brezinski, James G. Fujimoto
      Pages 217-233
    6. Daniel H. Turnbull
      Pages 235-243
    7. Kersti K. Linask, James C. Huhta
      Pages 245-252
    8. Diane C. Slusarski, Victor G. Corces
      Pages 253-261
  6. Cell Lineage Analysis

    1. Front Matter
      Pages 277-277
    2. Magdalena Zernicka-Goetz, Jonathon Pines
      Pages 279-287
    3. Seong-Seng Tan, Leanne Godinho, Patrick P. L. Tam
      Pages 289-295
    4. Robert G. Gourdie, Gang Cheng, Robert P. Thompson, Takashi Mikawa
      Pages 297-304
    5. Diana K. Darnell, Virginio Garcia-Martinez, Carmen Lopez-Sanchez, Shipeng Yuan, Gary C. Schoenwolf
      Pages 305-321
    6. Paul J. Heid, Jeff Hardin
      Pages 323-330
    7. Sally A. Moody
      Pages 331-347
  7. Chimeras

    1. Front Matter
      Pages 365-365
    2. Diana K. Darnell, Gary C. Schoenwolf
      Pages 367-371
    3. Nicole M. Le Douarin, Françoise Dieterlen-Lièlvre, Marie-Aimèe Teillet, Catherine Ziller
      Pages 373-386
    4. Elizabeth E. LeClair, Rocky S. Tuan
      Pages 387-396
    5. Richard L. Gardner, Timothy J. Davies
      Pages 397-424
    6. Simon J. Kinder, Seong-Seng Tan, Patrick P. L. Tam
      Pages 425-437
    7. Margaret L. Kirby, Harriett Stadt, Donna Kumiski, Vlad Herlea
      Pages 439-442
    8. Josiane Fontaine-Pérus
      Pages 443-446
    9. John Yochem, Meera Sundaram, Elizabeth A. Bucher
      Pages 447-462
  8. Experimental Manipulation of Embryos

    1. Front Matter
      Pages 463-463

About this book


The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this first volume of three presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors.
The second and third volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.


Drosophila Gastrulation Mesoderm Vertebrate developmental biology evolution evolutionary biology genes physiology

Editors and affiliations

  • John M. Walker
  • Rocky S. Tuan
    • 1
  • Cecilia W. Lo
    • 2
  1. 1.Thomas Jefferson UniversityPhiladelphia
  2. 2.University of PennsylvaniaPhiladelphia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-852-3
  • Online ISBN 978-1-59259-685-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors