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© 2004

Molecular Diagnosis of Infectious Diseases

  • Jochen Decler
  • Udo Reischl
Book

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 94)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Protein-Based Identification Of Antigens

    1. Front Matter
      Pages 1-1
    2. Karen M. Dobos, John S. Spencer, Ian M. Orme, John T. Belisle
      Pages 3-17
    3. Alexander Krah, Peter R. Jungblut
      Pages 19-32
    4. Stephen Thompson
      Pages 33-45
  3. DNA-Based Identification of Antigens

    1. Front Matter
      Pages 47-47
    2. Lucas D. Bowler
      Pages 49-66
    3. Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, Raffaele A. Calogero
      Pages 67-90
    4. Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, Yasir A. W. Skeiky
      Pages 91-106
  4. Identification of Epitopes and Immunomodulatory Components

    1. Front Matter
      Pages 107-107
    2. Christine Hager-Braun, Kenneth B. Tomer
      Pages 109-120
    3. Timothy W. Tobery, Michael J. Caulfield
      Pages 121-132
    4. Ludwig Deml, Jens Wild, Ralf Wagner
      Pages 133-157
    5. Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, Wulf Schneider-Brachert
      Pages 159-175
  5. Expression of Recombinant Proteins

  6. Purification, Modification, and Renaturation of Recombinant Proteins

About this book

Introduction

In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references.
Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

Keywords

infectious diseases

Editors and affiliations

  • Jochen Decler
    • 1
  • Udo Reischl
    • 2
  1. 1.Accenture GmbHMunichGermany
  2. 2.Institute for Medical Microbiology and HygieneUniversity of RegensburgRegenburgGermany

Bibliographic information

  • Book Title Molecular Diagnosis of Infectious Diseases
  • Editors Jochen Decker
    Udo Reischl
  • Series Title Methods in Molecular Medicine™
  • DOI https://doi.org/10.1385/1592596797
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-58829-221-6
  • Softcover ISBN 978-1-61737-430-2
  • eBook ISBN 978-1-59259-679-9
  • Series ISSN 1543-1894
  • Series E-ISSN 1940-6037
  • Edition Number 2
  • Number of Pages XVI, 464
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Infectious Diseases
  • Buy this book on publisher's site
Industry Sectors
Pharma
Health & Hospitals
Biotechnology
Public Health
Internal Medicine & Dermatology

Reviews

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics. Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. "-Doody's Health Sciences Book Review Journal

"...Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book."
-Archives of Pathology and Laboratory Medicine

"...a valuable methodological book for molecular biologists in a hospital laboratory or in academia." -Trends in Parasitology

"...a powerful tool for developing better experiments in the diagnostics of infectious diseases." -International Microbiology

"...a very good compilation of techniques that can be used for numerous applications."
-Analytical Biochemistry

"...a useful reference source for anyone who wants to manipulate microbial protein for diagnostic purposes."
-International Journal of Medical Microbiology

"...this book continues the well-established format of this series from Humana Press in providing clearly described laboratory protocols with useful background discussion...recommended as a useful laboratory reference book..." - Proteomics

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics." -Doody's Health Sciences and Book Review Journal

"...well-organized...provides detailed methods and practical tips...the book can be recommended for students, graduate students, and postdoctoral, fellows in the field of proteomics..." - Clinical Infectious Diseases

"...brings together the key elements of oral medicine and nutrition in an accessible form for the dental professional...an excellent reference text...wholeheartedly recommend(ed)." - American Society for Clinical Nutrition

Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source." -Archives of Pathology and Laboratory Medicine