Table of contents

  1. Front Matter
    Pages i-xiv
  2. Chromosomal Techniques

    1. Front Matter
      Pages 1-1
    2. Nicoletta Archidiacono, Rosalia Marzella, Cosma Spalluto, Margherita Pennacchia, Luigi Viggiano, Mariano Rocchi
      Pages 3-13
    3. Henry H. Q. Heng, Barbara Spyropoulos, Peter B. Moens
      Pages 15-27
    4. Robert D. C. Saunders
      Pages 103-113
    5. Stephen A. Wilcox, Roland Toder, Pino Maccarone, Jennifer A. Marshall-Graves
      Pages 115-127
  3. Tissue Techniques

    1. Front Matter
      Pages 129-129
    2. Tim D. Hewitson, Teresa Bisucci, Ian A. Darby
      Pages 157-164
    3. Malcolm D. Smith, Angela Parker, Riyani Wikaningrum, Mark Coleman
      Pages 165-175
    4. Ernst J. M. Speel, Anton H. N. Hopman, Paul Komminoth
      Pages 195-216
    5. Ross F. Waller, Geoffrey I. McFadden
      Pages 259-277
    6. Murray Hargrave, Peter Koopman
      Pages 279-289
    7. Teresa Bisucci, Tim D. Hewitson, Ian A. Darby
      Pages 291-303
  4. Applications

    1. Front Matter
      Pages 305-305
    2. William G. Kearns, Myung-Geol Pang, Darren Griffin, Lesil Brihn, Michael Stacey, Gustavo F. Doncel et al.
      Pages 307-321
  5. Back Matter
    Pages 339-343

About this book


InIn Situ Hybridization Protocols, 2nd Edition, Ian Darby updates the highly successful 1st edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques-many not covered in the first edition-and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Each protocol provides detailed, easy-to-follow instructions, along with realistic commentaries on how to vary their application and how to troubleshoot problems that arise.
Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire .


DNA FISH PCR RNA Tumor apoptosis biology chromosome electron microscopy gene expression genes hybridization microscopy molecular biology tissue

Editors and affiliations

  • Ian A. Darby
    • 1
  1. 1.RMIT University BundooraVictoriaAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-686-4
  • Online ISBN 978-1-59259-677-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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