Protein Purification Protocols

  • Paul Cutler

Part of the Methods in Molecular Biology book series (MIMB, volume 244)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Shawn Doonan, Paul Cutler
    Pages 1-13
  3. J. Mark Skehel
    Pages 15-20
  4. Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, Peter R. Shewry
    Pages 21-27
  5. Anne F. McGettrick, D. Margaret Worrall
    Pages 29-35
  6. Paul D. Bridge, Tetsuo Kokubun, Monique S. J. Simmonds
    Pages 37-46
  7. Alyson K. Tobin, Caroline G. Bowsher
    Pages 53-63
  8. Robert J. Beynon, Simon Oliver
    Pages 75-84
  9. Shawn Doonan
    Pages 85-90
  10. Shawn Doonan
    Pages 91-99
  11. Chris Selkirk
    Pages 125-131
  12. Paul A. O’Farrell
    Pages 133-138
  13. Paul Cutler
    Pages 139-149
  14. Anne F. McGettrick, D. Margaret Worrall
    Pages 151-157
  15. Iris West, Owen Goldring
    Pages 159-166
  16. Paul Cutler
    Pages 167-177

About this book


In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations.
Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.

Editors and affiliations

  • Paul Cutler
    • 1
  1. 1.Genomic and Proteomic Sciences Medicines Research CenterGlaxoSmithKline PharmaceuticalsStevenageUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-067-0
  • Online ISBN 978-1-59259-655-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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