Atomic Force Microscopy

Biomedical Methods and Applications

  • Pier Carlo Braga
  • Davide Ricci

Part of the Methods in Molecular Biology™ book series (MIMB, volume 242)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. The Basics of Atomic Force Microscopy

    1. Front Matter
      Pages 1-1
    2. Davide Ricci, Pier Carlo Braga
      Pages 3-12
    3. Davide Ricci, Pier Carlo Braga
      Pages 13-23
    4. Davide Ricci, Pier Carlo Braga
      Pages 25-37
    5. Martin Hegner, Youri Arntz
      Pages 39-49
  3. Morphostructural Analysis of Cellular Structures

    1. Front Matter
      Pages 51-51
    2. Gillian R. Bushell, Colm Cahill, Sverre Myhra, Gregory S. Watson
      Pages 53-67
    3. Stylliani Lydataki, Miltiadis K. Tsilimbaris, Eric S. Lesniewska, Alain Bron, Iannis G. Pallikaris
      Pages 69-83
    4. Gerlinde Bischoff, Anke Bernstein, David Wohlrab, Hans-Joachim Hein
      Pages 105-124
    5. Davide Ricci, Massimo Grattarola, Mariateresa Tedesco
      Pages 125-140
    6. Grayson W. Marshall Jr., Sally J. Marshall, Mehdi Balooch, John H. Kinney
      Pages 141-159
    7. Jason J. Davis, Trevor Powell, H. Allen O. Hill
      Pages 161-178
  4. Subcellular Structures Investigation

    1. Front Matter
      Pages 189-189
    2. Luis Felipe Jiménez-García, María de Lourdes Segura-Valdez
      Pages 191-199
    3. Olga I. Kiselyova, Igor V. Yaminsky
      Pages 217-230
    4. Kaushik Nag, Robert R. Harbottle, Amiyo K. Panda, Nils O. Petersen
      Pages 231-243
    5. Stefan Thalhammer, Pietro Gobbi, Mirella Falconi, Giovanni Mazzotti, Wolfgang M. Heckl
      Pages 245-254
    6. Stefan W. Schneider, Rainer Matzke, Manfred Radmacher, Hans Oberleithner
      Pages 255-279
    7. Peter R. Smith, Dale J. Benos
      Pages 281-289
    8. Dimitrios Fotiadis, Andreas Engel
      Pages 291-303
  5. Functional Investigations with AFM

    1. Front Matter
      Pages 305-305
    2. Ricardo de Souza Pereira
      Pages 315-321
    3. Settimio Grimaldi, Marco Girasole, Antonio Cricenti
      Pages 323-339
    4. Justin Legleiter, Tomasz Kowalewski
      Pages 349-364
    5. Martin Hegner, Wilfried Grange, Patricia Bertoncini
      Pages 369-381
  6. Back Matter
    Pages 383-394

About this book


Although atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.
Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own work.

Editors and affiliations

  • Pier Carlo Braga
    • 1
  • Davide Ricci
    • 2
  1. 1.Department of Pharmacology School of MedicineUniversity of MilanMilanItaly
  2. 2.Department of Biophysical and Electronic EngineeringUniversity of GenoaGenoaItaly

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-094-6
  • Online ISBN 978-1-59259-647-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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